EVALUATION OF THE ANTIOXIDANT ACTIVITIES, TOTAL PHENOLIC AND FLAVONOID CONTENTS OF MELIENTHA SUAVIS PIERRE
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Abstract
Melientha suavis Pierre (pak-wan pa) is a wild food plant found in Thailand and Southeast Asia. The aim of this research was to examine its antioxidant activities, total phenolic and flavonoid contents of pak-wan pa in Thailand. Pak-wan pa extracts were prepared by solvent extraction using soxhlet apparatus. The antioxidant activities of the extracts were determined using 2,2-diphenyl-1-picryhydrazyl radical scavenging (DPPH) assay and ferric reducing antioxidant power (FRAP) assay. The results found that the water extract of pak-wan pa exhibited antioxidant activity with an IC50 of 900-1150 μg/mL in DPPH assay and 20 μg/mL in FRAP assay, compared with that of gallic acid (2.84+0.008 μg/mL) and trolox (0.94 ± 0.224), respectively. The highest total phenolic and flavonoid contents of pak-wan pa was found in the methanolic extract (265-1,978 mgGE/100 g dry extract and 21-459 mgTE/100g dry extract) and in water extract (183-296 and 17-20 mgCE/100g dry extract), respectively. The results also showed a strong correlation between the FRAP scavenging activities and the flavonoid contents (Pearson correlation = 1.000) with significantly difference (p-value=0.013).
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