RAPD MARKER FOR DETERMINATION OF PHYLOGENETIC RELATIONSHIPS OF 15 CURCUMA SPECIES FROM THAILAND
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Abstract
Curcuma, a rhizomatous herb belonging to the family Zingiberaceae, has been used as a natural food additive, cosmetic and folk medicine in Thailand. According to the similar morphology of Curcuma species, makes it difficult for species identification. In this study, Random Amplified Polymorphic DNA (RAPD) was employed for determination of the phylogenetic relationships among 15 Curcuma species from Thailand. Out of thirty random deca-arbitrary primers, only four produced clear and reproducible polymorphic bands. Twenty-two to twenty-eight products were amplified, with an average of 24.5 bands by each primer. A total of 98 bands ranging from 208 to 4136 base pairs in size were amplified, among which 39 products were found to be polymorphic. The similarity index (SI) ranged from 0.0909-0.9222. The dendrograms were constructed based on unweighted pair group method with arithmetic averages (UPGMA). The results from the cluster diagram could be divided into three major groups and the phylogenetic relationships were correlated with the morphological characteristics. In conclusion, RAPD marker was successfully for differentiating among 15 Curcuma species from Thailand and providing a simple and rapid tool for differentiation.
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References
Angel G, Makeshkumar T, Mohan C, Vimala B and Nambinsan B. 2008. Genetic diversity analysis of starchy Curcuma species using RAPD markers. Journal of Plant Biochemistry and Biotechnology. 17: 173-176.
Cao H, Sasaki Y, Fushimi H and Komatsu K. 2001. Molecular analysis of medicinally-Used Chinese and Japanese Curcuma based on 18S rRNA gene and trnK Gene sequences. Biological & Pharmaceutical Bulletin. 24:1389-1394.
Heubl G. 2010. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. Planta Medica. 76:1963-1974.
Kitamura C, Nagoe T, Prana M, Agusta A, Ohashi K and Shibuya H. 2007. Comparison of Curcuma sp. in Yakushima with C. aeruginosa and C. zedoaria in Java by trnK gene sequence, RAPD pattern and essential oil component. Journal of Natural Medicines. 61:239-243.
Nei M and Li W. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of American. 76:5269-5273.
Pojanagaroon S, Praphet R, Kaewrak C and Yotdi A. 2004. Characterization of Krachai-Dam (Kaempferia parviflora) cultivars using RAPD markers, morphological traits and chemical components of essential oil from rhizomes. Proceedings of the 42nd Kasetsart University Annual Conference, Kasetsart, Thailand. 56-65.
Ravindran P, Babu KN and Shiva K. 2007. Botany and crop improvement of Turmeric. In: Ravindran PN, Nirmal BK and Sivaraman K, editors. Turmeric The genus Curcuma. Boca Raton: CRC Press; pp. 15-70.
Sasikumar, B. 2005. Genetic resources of Curcuma: diversity, characterization and utilization. Plant Genetic Resources. 3:230-251.
Semagn K, Bjornstad A and Ndjiondjop M. 2006. An overview of molecular marker methods for plants. African Journal of Biotechnology. 5:2540-2568.
Sirirugsa P, Larsen K and Maknoi C. 2007. The genus Curcuma L. (Zingiberaceae): distribution and classification with reference to species diversity in Thailand. Gardens' Bulletin Singapore. 59:203-220.
Skornickova J. 2006. Curcuma - Stunning beauty, hidden treasure. GardenWise. 2-5.
Syamkumar S and Sasikumar B. 2007. Molecular marker based genetic diversity analysis of Curcuma species from India. Scientia Horticulturae. 112:235-241.
Vanijajiva O, Sirirugsa P and Suvachittanont W. 2005. Confirmation of relationships among Boesenbergia (Zingiberaceae) and related genera by RAPD. Biochemical Systematics and Ecology. 33:159-170.
Williams J, Kubelik A, Livak K, Rafalski J and Tingey S. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research.18:6531-6535.
Zou X, Dai Z, Ding C, Zhang L, Zhou Y and Yang R. 2011. Relationships among six medicinal species of Curcuma assessed by RAPD markers. Journal of Medicinal Plants Research. 5:1349-1354.
Zaveska E, Fer T, Sida O, Krak K, Marhold K and Leong-Skornickova J. 2012. Phylogeny of Curcuma (Zingiberaceae) based on plastid and nuclear sequences: Proposal of the new subgenus Ecomata. TAXON. 61:747-63.