CYTOTOXIC, ANTIPROLIFERTIVE AND APOPTOTIC ACTIVITY OF SECONDARY METABOLITES FROM ASPERGILLUS UNQUIS CRI282-03 ON HUMAN COLON CANCER CELL LINE (COLO 205)

Article Sidebar

PDF
Published: Oct 5, 2023
Keywords:
spergillus unguis CRI282-03 nidulin aspergilusidone D unguinol antiproliferative activity

Main Article Content

Nalinee Pradubyat
Department of Pharmacology, Faculty of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand
Suchada Jongrungruangchok
epartment of Pharmaceutical chemistry, Faculty of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand
Thanapat Songsak
Department of Pharmacognosy, Faculty of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand
Supakit Wongwiwatthananukit
Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, HI

Abstract

The purpose of this study is to study the antiproliferative and cytotoxic effect of  three secondary metabolites which are nidulin, aspergillusidone D, and unguinol from marine-derived fungi Aspergillus unguis CRI282-03 on colon cancer cells (COLO 205). In the course of a microbial screening process, A. unguis CRI282-03 was isolated from soil samples collected at the Royal Navy Base in Tub-La-Mu bay, Pang-nga Province, Thailand. The isolated fungus of interest was cultivated in Chulabhorn Research Institute (CRI). Nidulin, aspergillusidone D and unguinol were tested on COLO 205 cell. In addition, the cytotoxic activity of those three pure constituents were determined using MTT assay, the antiprolifertive as well as the anti-apoptotic activity were evaluated by flow cytometry method. The result from MTT assay showed that IC50 value of nidulin, aspergillusidone D, and unguinol on COLO 205 cells were 15, 21 and 33 µM, respectively. Furthermore, nidulin provided anticancer activity through inducing apoptosis to COLO 205. From the performed assay nidulin showed greater antiproliferation activity than aspergillusidone D.

Article Details

How to Cite
1.
Pradubyat N, Jongrungruangchok S, Songsak T, Wongwiwatthananukit S. CYTOTOXIC, ANTIPROLIFERTIVE AND APOPTOTIC ACTIVITY OF SECONDARY METABOLITES FROM ASPERGILLUS UNQUIS CRI282-03 ON HUMAN COLON CANCER CELL LINE (COLO 205). Interprof J Health Sci [Internet]. 2023 Oct. 5 [cited 2024 Sep. 17];14(1):77-92. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/136
Issue
Vol. 14 No. 1 (2016): January - June
Section
Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Journal of TCI is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence, unless otherwise stated. Please read our Policies page for more information.

References

American Cancer Society; Cancer Facts and Figures for African Americans 2013-2014. Atlanta. Accessed online March. 25, 2014. www.cancer.org/research/cancerfactsstatistics/cancerfactsfigures 2014

Berridge, M.V., Herst, P.M., Tan, A.S. 2005. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annual Rev. 11: 127-152.

Cancer Facts and statistics. Available at: www.cancer.org/research/cancerfactsstatistics/accessed on October 14, 2013.

Daigle, S.R., Olhava, E.J., Therkelsen, C.A., Majer, C.R., Sneeringer, C.J., Song, J., et al. 2011. Selective Killing of Mixed Lineage Leukemia Cells by a Potent Small-Molecule DOT1L Inhibitor. Cancer Cell 20: 53–65.

Jongrungruangchok, S., Tanasupawat, S., Kitakoop, P. 2008. Bioactive compound from Penicillium sp CRIM 301. Proceedings of RSU Research Conference. 3 Apr. p. 8-16.

Lee, J.C., Yu, M.K., Lee, R., Lee, Y.H., Jeon, J.G., Lee, M.H., et al. 2008. Terrein reduces pulpal inflammation in human dental pulp cells. J Endod. 34:433-7.

Newman, D.J., Cragg, G.M. and Snader, K.M. 2003. Natural products as sources of new drugs over the period 1981-2002. J Nat Prod. 66, 1022-1037.

Park, S.H., Kim, D.S., Kim, W.G., Ryoo, I.J., Lee, D.H., Huh, C.H., et al. 2004. Terrein: a new melanogenesis inhibitor and its mechanism. Cell Mol Life Sci. 61:2878-85.

Parvatkar, R.R., D'Souza, C., Tripathi, A., Naik, C.G. 2009. Aspernolides A and B, butenolides from a marine-derived fungus Aspergillus terreus. Phytochemistry 70:128-32.

Porameesanaporn, Y., Uthaisang-Tanechpongtamb, W., Jarintanan, F., Jongrungruangchok, S., Thanomsub Wongsatayanon, B. 2013. Terrein induces apoptosis in Hela human cervical carcinoma cells through p53 and ERK regulation. Oncol Rep. 29(4):1600-8.

Pradubyat, N., Jongrungruangchok, S., Songsak, T. Bioactive Constituents from Aspergillus unguis CRI282-03. Oral presentation of the 2nd International Conference on Advanced Pharmaceutical Research Strategies and Innovation in Pharmaceutical Reseach: Safety, Efficacy and Quality. 12 Mar, 2015. p. 22.

Sriplung, H. 2003. Projection of cancer problems. In: Sriplung, H. Sontipong, S. Martin, N. et al. Cancer in Thailand Vol. III. 1995-1997, Bangkok, National.

Sureram, S., Kesornpun, C., Mahidol, C., Ruchirawat, S., and Kittakoop, P. 2013. Directed biosynthesis through biohalogenation of secondary metabolites of the marine-derived fungus Aspergillus unguis. RSC Adv.7 3, 1781-1788.

Sureram, S., Wiyakrutta, S., Ngamrojanavanich, N., Mahidol, C., Ruchirawat, S., and Kittakoop, P. 2012. Depsidones, aromatase inhibitors and radical scavenging agents from the marine-derived fungus Aspergillus unguis CRI282-03. Planta Med. 78(6): 582-588.

World Health Organization. 2010. The top 10 causes of death - World Health Organization. Accessed online October. 20, 2013. www.who.int/mediacentre/factsheets/fs310.pdf. accessed on December 11, 2013.

Yu, M.K., Lee, J.C., Kim, J.H., Lee, Y.H., Jeon, J.G., Jhee, E.C., et al. 2009. Anti-inflammatory effect of peroxisome proliferator activated receptor gamma on human dental pulp cells. J Endod 35:524-528.