CYTOTOXIC, ANTIPROLIFERTIVE AND APOPTOTIC ACTIVITY OF SECONDARY METABOLITES FROM ASPERGILLUS UNQUIS CRI282-03 ON HUMAN COLON CANCER CELL LINE (COLO 205)

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Published: Oct 5, 2023
Keywords:
spergillus unguis CRI282-03 nidulin aspergilusidone D unguinol antiproliferative activity

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Nalinee Pradubyat
Department of Pharmacology, Faculty of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand
Suchada Jongrungruangchok
epartment of Pharmaceutical chemistry, Faculty of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand
Thanapat Songsak
Department of Pharmacognosy, Faculty of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand
Supakit Wongwiwatthananukit
Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, HI

Abstract

The purpose of this study is to study the antiproliferative and cytotoxic effect of  three secondary metabolites which are nidulin, aspergillusidone D, and unguinol from marine-derived fungi Aspergillus unguis CRI282-03 on colon cancer cells (COLO 205). In the course of a microbial screening process, A. unguis CRI282-03 was isolated from soil samples collected at the Royal Navy Base in Tub-La-Mu bay, Pang-nga Province, Thailand. The isolated fungus of interest was cultivated in Chulabhorn Research Institute (CRI). Nidulin, aspergillusidone D and unguinol were tested on COLO 205 cell. In addition, the cytotoxic activity of those three pure constituents were determined using MTT assay, the antiprolifertive as well as the anti-apoptotic activity were evaluated by flow cytometry method. The result from MTT assay showed that IC50 value of nidulin, aspergillusidone D, and unguinol on COLO 205 cells were 15, 21 and 33 µM, respectively. Furthermore, nidulin provided anticancer activity through inducing apoptosis to COLO 205. From the performed assay nidulin showed greater antiproliferation activity than aspergillusidone D.

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1.
Pradubyat N, Jongrungruangchok S, Songsak T, Wongwiwatthananukit S. CYTOTOXIC, ANTIPROLIFERTIVE AND APOPTOTIC ACTIVITY OF SECONDARY METABOLITES FROM ASPERGILLUS UNQUIS CRI282-03 ON HUMAN COLON CANCER CELL LINE (COLO 205). Interprof J Health Sci [Internet]. 2023 Oct. 5 [cited 2024 Nov. 24];14(1):77-92. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/136
Issue
Vol. 14 No. 1 (2016): January - June
Section
Research Articles
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