DETERMINATION OF SWERTIAMARIN CONTENT BY TLC-DENSITOMETER IN FAGRAEA FRAGRANS ROXB. LEAVES
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Abstract
Fagraea fragrans Roxb. (Gentianaceae) is one of the most common herbs in Thai traditional medicine. Various parts of the plant have been used for the treatment of malaria, asthma, fever, and skin eruption. The major component of F. fragrans leaves is swertiamarin, a secoiridoid glycoside. Chromatographic separation of swertiamarin in ethanolic extract was performed on silica gel 60 F254 TLC plates, using the mobile phase of dichloromethane : methanol (10:2, v/v). Detection of swertiamarin was done by densitometric scanning at 240 nm. The identity of the swertiamarin band in the sample extract was confirmed by overlapping the UV absorption spectrum of the sample with that of the reference standard swertiamarin. The linearity range of swertiamarin by TLC-densitometry was 0.3- 1.8 µg/spot, (R2) of 0.9995. The precisions calculated by the %RSD of repeatability and intermediate precision, were 0.95 and 1.94 respectively. The average recovery was 101.3. LOD and LOQ were 9.71 ng/band and 29.44 ng/band respectively.
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