ANTIBACTERIAL ACTIVITY AND FORCED DEGRADATION STUDY OF CAESALPINIA SAPPAN L. HEARTWOOD EXTRACT FOR INHIBITING PUS-FORMING BACTERIA

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Sukanya Settharaksa
Pathamaporn Pathompak
Fameera Madaka
Chaowalit Monton

Abstract

The aim of this research was to examine the antibacterial activity of Caesalpinia sappan heartwood extract against pus-forming bacteria. Furthermore, forced degradation of the extract was also investigated. C. sappan heartwood extract was prepared by maceration using 95% ethanol and decoction using boiling water. The antibacterial activity of extract was determined against Staphylococcus aureus TISTR 1466, Staphylococcus epidermidis TISTR 518 and Propionibacterium acne DMST 14916. The result found that the 95% ethanol extract of C. sappan inhibited bacterial growth with an inhibition zone of 20.40±1.64, 33.37±0.51, and 34.48±2.01 mm, respectively. The result found that the boiling water extract inhibited bacterial growth with an inhibition zone of 21.40±0.17, 30.40±0.00, and 57.90±1.20 mm, respectively. The forced degradation test found that 95% ethanol extract was decreased the antibacterial activity by neutral hydrolysis, acid hydrolysis, and thermal hydrolysis. In the case of boiling water extract, decreasing of antibacterial activity found in neutral hydrolysis, acid hydrolysis, and alkaline hydrolysis. In summary, C. sappan extract showed potent antibacterial activity. However, some condition caused decreasing of antibacterial activity should be avoided. 

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How to Cite
1.
Settharaksa S, Pathompak P, Madaka F, Monton C. ANTIBACTERIAL ACTIVITY AND FORCED DEGRADATION STUDY OF CAESALPINIA SAPPAN L. HEARTWOOD EXTRACT FOR INHIBITING PUS-FORMING BACTERIA. Interprof J Health Sci [Internet]. 2023 Oct. 5 [cited 2024 Dec. 6];14(2):64-9. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/151
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Research Articles

References

Bae IK, Min HY, Han AR, Seo EK, Lee SK. 2005. Suppression of lipopolysaccharide-induced expression of inducible nitric oxide synthase by brazilin in RAW 264.7 macrophage cells. Eur J Pharmacol. 513(3): 237-242.

Goossens H. 2000. Antibiotic resistance and policy in Belgium. Verh K Acad Geneeskd Belg. 62: 439-469.

Guyot S, Guerneve CL, Marnet N, Drilleau JF. 1999. Plant Polyphenol 2. Plenum Publisher, New York, pp. 211-222.

Harjit K, Amini MH, Sutee A. 2016. Evaluation of antioxidation and anthelmintic properties of Caesalpinia sappan L leaves. IJPPR. 8(2): 362-368.

Nilesh PN, Mithun SR, Rangabhatla GSV, Meharaj A. 2015. Brazilin from Caesalpinia sappan heartwood and its pharmacological activities: A review. APJTM. 8(6): 421-430.

Seanjum C, Chaiyasut C, Kadchumsang S, Chansakaow S, Suttajit M. 2010. Antioxidant activity and protective effects on DNA damage of Caesalpinia sappan L. extract. J Medic Plants Res. 4(15): 1594-1600.

Sireeratawong S, Piyabhan P, Singhalak T, Wongkrajang Y, Temsiririrkkul R, Punsrirat J. 2010. Toxicity evaluation of Caesalpinia sappan wood in rats. J Med Assoc Thai. 93(7): 50-57.

Srilakshmi VS, Vijayan P, Raj PV, Dhanaraj SA, Chandrashekhar HR. 2010. Hepatoptotective properties of Caesalpinia sappan Linn. Heartwood on carbon tetrachloride induce toxicity. Ind J Exper Biol. 48(9): 905-910.

Wetwitayaklung P, Phaechamud T, Keokitichai S. 2005. The antioxidant activity of Caesalpinia sappan L. heartwood in various ages. Naresuan Uni J. 13(2): 43-52.

Xu HX, Lee SF. 2004. The antibacterial principle of Caesalpinia sappan. Phytother Res. 18: 647-651.