ANTIBACTERIAL ACTIVITY AND FORCED DEGRADATION STUDY OF CAESALPINIA SAPPAN L. HEARTWOOD EXTRACT FOR INHIBITING PUS-FORMING BACTERIA

Abstract

The aim of this research was to examine the antibacterial activity of Caesalpinia sappan heartwood extract against pus-forming bacteria. Furthermore, forced degradation of the extract was also investigated. C. sappan heartwood extract was prepared by maceration using 95% ethanol and decoction using boiling water. The antibacterial activity of extract was determined against Staphylococcus aureus TISTR 1466, Staphylococcus epidermidis TISTR 518 and Propionibacterium acne DMST 14916. The result found that the 95% ethanol extract of C. sappan inhibited bacterial growth with an inhibition zone of 20.40±1.64, 33.37±0.51, and 34.48±2.01 mm, respectively. The result found that the boiling water extract inhibited bacterial growth with an inhibition zone of 21.40±0.17, 30.40±0.00, and 57.90±1.20 mm, respectively. The forced degradation test found that 95% ethanol extract was decreased the antibacterial activity by neutral hydrolysis, acid hydrolysis, and thermal hydrolysis. In the case of boiling water extract, decreasing of antibacterial activity found in neutral hydrolysis, acid hydrolysis, and alkaline hydrolysis. In summary, C. sappan extract showed potent antibacterial activity. However, some condition caused decreasing of antibacterial activity should be avoided.