ESSENTIAL OIL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF PIPER RIBESOIDES LEAVES ENDEMIC TO THAILAND
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Abstract
The essential oil obtained by hydrodistillation on Piper ribesoides dried leaves was analyzes by Gas Chromatography-Mass Spectrometry (GC-MS). The major constituents were β-bisabolene (37.63%), followed by α-curcumene (17.58%), and β-caryophyllene (13.01%). According to biological activities, P. ribesoides essential oil was screened for antioxidant activity by DPPH radical scavenging method. The result showed 100 μg/ml of this essential oil exhibited the inhibitory activity of 36.30±1.68%. This essential oil also exhibited antimicrobial activity against Candida albicans with the inhibition zones about 15 mm at the concentration of 10 mg/ml, followed by gram positive bacteria (Staphylococcus aureus, Bacillus subtilis, and Micrococcus luteus) with the inhibition zones of 7-9 mm at the concentration of 10 mg/ml, and against gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa) with the inhibition zones of 7-9 mm at the concentration of 100 mg/ml. In addition, this oil was non-toxic to brine shrimp with the LC50 value > 1,000 μg/ml.
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