ESSENTIAL OIL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF PIPER RIBESOIDES LEAVES ENDEMIC TO THAILAND

Main Article Content

Supawan Bunrathep
Suchada Jonrunruangchok
Nijsiri Ruangrungsi

Abstract

The essential oil obtained by hydrodistillation on Piper ribesoides dried leaves was analyzes by Gas Chromatography-Mass Spectrometry (GC-MS).  The major constituents were β-bisabolene (37.63%), followed by α-curcumene (17.58%), and β-caryophyllene (13.01%).  According to biological activities, P. ribesoides essential oil was screened for antioxidant activity by DPPH radical scavenging method.  The result showed 100 μg/ml of this essential oil exhibited the inhibitory activity of 36.30±1.68%.  This essential oil also exhibited antimicrobial activity against Candida albicans with the inhibition zones about 15 mm at the concentration of 10 mg/ml, followed by gram positive bacteria (Staphylococcus aureus, Bacillus subtilis, and Micrococcus luteus) with the inhibition zones of 7-9 mm at the concentration of 10 mg/ml, and against gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa) with the inhibition zones of 7-9 mm at the concentration of 100 mg/ml.  In addition, this oil was non-toxic to brine shrimp with the LC50 value > 1,000 μg/ml.

Article Details

How to Cite
1.
Bunrathep S, Jonrunruangchok S, Ruangrungsi N. ESSENTIAL OIL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF PIPER RIBESOIDES LEAVES ENDEMIC TO THAILAND. Interprof J Health Sci [Internet]. 2023 Oct. 5 [cited 2024 Dec. 6];14(2):77-85. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/153
Section
Research Articles

References

Farag RS, Daw ZY, Hewedi FM, El-Baroty GSA. 1989. Antimicrobial activity of some Egyptian spice essential oils. J Food Protect 52: 665-667.

Griffin GS, Markham LJ, Leach ND. 2000. An agar dilution method for the determination of the minimum inhibitory concentration of essential oils. J Essent Oil Res.12: 149-255.

Jirovetz L, Buchbauer G, Ngassoum MB, and Geissler M. 2002. Aroma compound analysis of Piper nigrum and Piper guineense essential oils from Cameroon using solid-phase microextraction – gas chromatography, solidphase microextraction – gas chromatogramphy – mass spectrometry and olfactometry. J Chromatography. A.976: 265–275.

Marjo K, Eija P, james ES. 2001. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and morphological difference of genotypes. Biochem Syst Ecol. 29: 267-285.

Martins AP, Salgueiro L, Vila R, Tomi, V, Canigueral S, Casanova J, Proenca Da Cunha A, and Adzet T. 1998. Essentail oils from four Piper species. Phytochemistry. 49(7): 2019-2013.

Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, Mclaughlin JL. 1982. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 45: 31-34.

Navickiene HMD, Morandim AA, Alecio AC, Regasini LO, Bergamo DCB, Telascrea M, Cavalheiro AJ, Lopes MN, Bolzani VS, Furlan M, Marques MOM, Young MCM, and Kato MJ. 2006. Composition and antifungal activity of essential oils from Piper aduncum, Piper arboreum and Piper tuberculatum. Quim Nova 29: 467-470.

Parra LA, Yhebra RS, Sardiñas IG, and Buela LI. (2001). Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts. Phytomed 8: 395-400.

Raju G and Maridass M. 2011. Composition, antifungal and cytotoxic activities of Essential oils of Piper barberi fruits. Int J Bio Technol 2(2): 100-105.

Rodrigues-Silva D, Endo EH, Filho BPD, Nakamura CV, Svidzinski TIE, Souza A, Young MCM, Ueda-Nakamura T, and Cortez DAG. 2009. Chemical composition and antimicrobial properties of Piper ovatum Vahl. Molecules 14(3): 1171-1182.

Salleh WMNHW, Farediah AHMAD F, Sirat HM, and Yen KH. 2012. Chemical compositions and antibacterial activity of the leaf and stem oils of Piper porphyrophyllum (Lindl.) N.E. BR. EXCLI J. 11: 399-406.

Salleh WMNHW, Farediah AHMAD F and Yen KH. 2014. Chemical composition of Piper stylosum Miq. and Piper ribesioides Wall. essential oils, and their antioxidant, antimicrobial and tyrosinase inhibition activities. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 13(5): 488-497

Schmidt E, Bail S, Friedl SM, Jirovetz L, Buchbauer G, Wanner J, Denkova Z, Slavchev A, Stoyanova A, Geissler M. 2010. Antimicrobial activity of single aroma compounds. Nat Prod Commun 5: 1365-1368.

Singh G, Sumitra M, Catalan C and Lampasona MP. 2004a. Chemical antioxidant and antifungal activities of volatile oil of black pepper and its acetone extract. J Sci Food Agric. 84: 1878-1884.

Sudmoon R, Tanee T, Wongpanich V, Bletter N, Chaveerach A. 2012. Ethnobotany and species specific molecular markers of some medicinal sakhan (Piper, Piperaceae). J Med Plants Res 6: 1168-1175.

Tundis R, Loizzo MR, Bonesi M, Menichini F, Mastellone V, Colica C, Menichini F. 2012. Comparative study on the antioxidant capacity and cholinesterase inhibitory activity of Citrus aurantifolia Swingle, C. aurantium L., and C. bergamia Risso and Poit. peel essential oils. J Food Sci 77: H40-H46.

Upadhyay RK and Jaiswal G. 2007. Evaluation of biological activities of Piper nigrum oil against Tribolium castaneum. Bull Insectology 60(1): 57-61

Vieira SCH, Paula LF, Svidzinski TIES, Filho BPD, Nakamura CV, Zouza a, Young MCM, and Cortez DAG. 2011. Antifungal activity of Piper diospyrifolium Kunth (Piperaceae) essential oil. Brazilian J Microbiol 42: 1001-1006.

Zouari N, Fakhfakh N, Zouari S, Bougatef A, Neffati M, Ayadi MA. 2011. Chemical composition, angiotensin I-converting enzyme inhibitory, antioxidant and antimicrobial activities of essential oil of Tunisian Thymus algeriensis Boiss. et Reut. (Lamiaceae). Food Bioprod Process 89: 257-265.