CHEMICAL CONSTITUENTS AND IN VITRO ANTIOXIDANT ACTIVITIES OF ESSENTIAL OIL FROM CURCUMA LEUCORRHIZA ROXB. RHIZOME

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Orawan Theanphong
Withawat Mingvanish
Thaya Jenjittikul

Abstract

The hydrodistillate essential oil from the fresh rhizomes of Curcuma leucorrhiza Roxb. was investigated for chemical constituents by gas chromatography-mass spectroscopy (GC-MS) and antioxidant activities by five different methods including DPPH radical scavenging, hydroxyl radical scavenging, hydrogen peroxide scavenging, ferrous ion chelating and ferric reducing power assays.  L-ascorbic acid and ethylenediaminetetraacetic acid (EDTA) were used as a positive control. The three major components in the essential oil were found to be germacrene D (21.65%), selina-3, 7(11)-diene (16.67%) and α-longinipine (11.16%). The EC50 value of the essential oil against hydroxyl radicals (10.72 ± 1.54 μg/ml) was found to be significantly higher than that of L-ascorbic acid (18.27 ± 1.54 μg/ml). The DPPH radical scavenging activity, hydrogen peroxide scavenging activity and ferric reducing power of the essential oil and L-ascorbic acid were found to be insignificantly different. The EC50 values of the essential oil and L-ascorbic acid were 8.88 ± 0.74 and 10.77 ± 0.97 μg/ml, respectively, for DPPH radical scavenging activity, 12.88 ± 1.38 and 11.04 ± 1.03 μg/ml, respectively, for hydrogen peroxide scavenging activity and 0.14 ± 0.02 and 0.10 ± 0.02 μg/ml, respectively, for ferric reducing power.  The essential oil showed less ferrous ion chelating activity with its EC50 value of 345.62 ± 13.29 μg/ml as compared with EDTA with its EC50 value of 154.19 ± 2.45 μg/ml.

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1.
Theanphong O, Mingvanish W, Jenjittikul T. CHEMICAL CONSTITUENTS AND IN VITRO ANTIOXIDANT ACTIVITIES OF ESSENTIAL OIL FROM CURCUMA LEUCORRHIZA ROXB. RHIZOME. Interprof J Health Sci [Internet]. 2023 Oct. 5 [cited 2024 Dec. 6];14(2):86-9. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/154
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Research Articles

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