PROTEIN KINASE INHIBITORY PROPERTIES OF EXTRACTS DERIVED FROM BOCCONIA FRUTESCENS AND GOMPHOCARPUS PHYSOCARPUS

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Published: Oct 5, 2023
Keywords:
Protein kinase inhibition hyphae formation inhibition assay occonia frutescens Gomphocarpus physocarpus

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Kūpa‘aikekaiao Thomas
Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, HI 96720, USA
Mengke Zhang
Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, HI 96720, USA
Justin Reinicke
Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, HI 96720, USA
Jimmy Parker
Early Detection Program Coordinator, Big Island Invasive Species Committee, 23 East Kawili Street, Hilo, HI 96720, USA
Donna Ohora
Department of Pharmacy Practice, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, Hilo, HI 96720, USA
Marisa M. Wall
United States Department of Agriculture, Agricultural Research Service, Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center, Hilo, HI 96720, USA
Thanapat Songsak
Department of Pharmacognosy, Faculty of Pharmacy, Rangsit University, Pathumtani 12000, Thailand
Supakit Wongwiwatthananukit
Department of Pharmacy Practice, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, Hilo, HI 96720, USA
Leng Chee Chang
Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, HI 96720, USA

Abstract

Bocconia frutescens is an invasive shrub originally introduced to Hawai’i as an ornamental plant. Gomphocarpus physocarpus is a shrub that is native to southern Africa, also introduced to Hawai’i. These two plants were selected for study due to the possibility of alkaloid contents and biological activities. The objective of this study was to examine the protein kinase inhibitory activity (PKI) of crude methanol extracts derived from Bocconia frutescens and Gomphocarpus physocarpus using hyphae formation inhibition assay against Streptomyces 85E at concentrations of 20 μg/disk. Extracts that showed protein kinase inhibitory  activity at this concentration were tested at lower concentrations. Results found that all B. frutescens extracts showed PKI activity, whereas none of the G. physocarpus showed biological activity. In addition, the presence of chelerythrine and berberine alkaloids in  B. frutescens  extracts were assessed during this study using thin layer chromatography (TLC) and liquid chromatography-massspectrometry (LC-MS) analysis. The results showed that both alkaloids were present in all B. frutescens extracts.

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1.
Thomas K, Zhang M, Reinicke J, Parker J, Ohora D, Wall MM, Songsak T, Wongwiwatthananukit S, Chang LC. PROTEIN KINASE INHIBITORY PROPERTIES OF EXTRACTS DERIVED FROM BOCCONIA FRUTESCENS AND GOMPHOCARPUS PHYSOCARPUS. Interprof J Health Sci [Internet]. 2023 Oct. 5 [cited 2024 Nov. 22];15(1):47-58. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/163
Issue
Vol. 15 No. 1 (2017): January - June
Section
Research Articles
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References

Bakal CJ, Davies JE. 2000. No longer an exclusive club: eukaryotic signaling domains in bacteria. Trends Cell Biol. 10(1): 32-38.

Bakal CJ, Vujaklija D, Davies JE. 2001. Immunochemical identification of a Stat3 analogue in Streptomycetes. Food Technology and Biotechnology. 39(4): 313-7.

Benitez DM, Saulibio D. 2007. Bocconia frutescens distribution on the Island of Hawai`i. https://www.researchgate.net/publication/29741022_bocconia_frutescens_distribution_on_the_island_of_hawaii (accessed June 20, 2016).

Chmura SJ, Dolan ME, Cha A, Mauceri HJ, Kufe DW, Weichselbaum RR. 2000. In vitro and in vivo activity of protein kinase C inhibitor chelerythrine chloride induces tumor cell toxicity and growth delay in vivo. Clinical Cancer Research. 6(2): 737-42.

Liang XG, Wu F, Wang YJ, Fu Z, Wang Y, Feng Y, Liang S. 2014. Research on bitter components from Coptis chinensis based on electronic tongue. China Journal of Chinese Materia Medica. 39(17): 3326-9.

Madureira AM, Ramalhete C, Mulhovo S, Duarte A, Ferreira, MU. 2011. Antibacterial activity of some African medicinal plants used traditionally against infectious diseases. Pharmaceutical Biology 50(4): 481-9.

Munsamy A & Naidoo Y. 2015. Laticifers in the leaves and stems of Gomphocarpus physocarpus: Distribution, structure and chemical composition. Planta Medica. 81(16): 155-61.

Nayeem AA, Khatun A, Rahman MS, Rahman M. 2011. Evaluation of phytochemical and pharmacological properties of Mikania cordata (Asteraceae) leaves. Journal of Pharmacognosy and Phytotherapy. 3(8): 118–123.

Quattrocchi U. 2012. CRC world dictionary of medicinal and poisonous plants: common names, scientific names, eponyms, synonyms, and etymology; CRC: Boca Raton, FL, 609.

Rojas-Sandoval J & Acevedo-Rodríguez P. 2016. Gomphocarpus physocarpus (balloon cotton bush). http://www.cabi.org/isc/datasheet/114618 (Accessed July 25, 2016).

Sánchez-Arreola E, Hernández-Molina LR, Sánchez-Salas JL, Martínez-Espino G. 2006. Alkaloids from Bocconia frutescens and biological activity of their extracts. Pharmaceutical Biology. 44(7): 540–3.

Shanbhag P, Bhave S, Vartak A, Kulkarni-Almeida A, Mahajan G. Villanueval I,

Davies J. 2015. Screening of microbial extracts for anticancer compounds using Streptomyces kinase inhibitor assay. Natural Products Communication. 10(7): 1287-91.

Stermitz FR, Lorenz P, Tawara JN, Zenewicz LA, Lewis K. 2000. Synergy in a medicinal plant: antimicrobial action of berberine potentiated by 5'-methoxyhydnocarpin, a multidrug pump inhibitor. Proceedings of the National Academy of Sciences. 97(4): 1433–7.

Son SY, Rhee HS, Lee MW, Park JM. 2014. Analysis of benzo[c]phenanthridine alkaloids in Eschscoltzia californica cell culture using HPLC-DAD and HPLC-ESI- MS/MS. Bioscience Biotechnology, Biochemistry. 78(7): 1103-11.

Su Y, Li S, Li N, Chen L, Zhang J, Wang J. 2011. Seven alkaloids and their antibacterial activity from Hypecoum erectum L. Journal of Medicinal Plants Research. 5(22): 5428-32.

Tavares LDC, Zanon G, Weber AD, Neto AT, Mostardeiro CP, Cruz IB, Oliveira RM, Ilha V, Dalcol II, Morel AF. 2014. Structure-activity relationship of benzophenanthridine alkaloids from Zanthoxylum rhoifolium having antimicrobial activity. PLoS ONE. 9(5): 1-10.

Waters B, Saxena G, Wanggui Y, Kau D, Wrigley S, Stokes R, Davies J. 2002. Identifying protein kinase inhibitors using an assay based on inhibition of aerial hyphae formation in Streptomyces. The Journal of Antibiotics. 55(4): 407-16.

Yu X, Gao X, Zhu Z, Cao Y, Zhang Q, Tu P, Chai X. 2014. Alkaloids from the tribe Bocconieae (Papaveraceae): A chemical and biological review. Molecules. 19(9): 13042–60.