PROTEIN KINASE INHIBITORY PROPERTIES OF EXTRACTS DERIVED FROM BOCCONIA FRUTESCENS AND GOMPHOCARPUS PHYSOCARPUS

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Published: Oct 5, 2023
Keywords:
Protein kinase inhibition hyphae formation inhibition assay occonia frutescens Gomphocarpus physocarpus

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Kūpa‘aikekaiao Thomas
Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, HI 96720, USA
Mengke Zhang
Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, HI 96720, USA
Justin Reinicke
Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, HI 96720, USA
Jimmy Parker
Early Detection Program Coordinator, Big Island Invasive Species Committee, 23 East Kawili Street, Hilo, HI 96720, USA
Donna Ohora
Department of Pharmacy Practice, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, Hilo, HI 96720, USA
Marisa M. Wall
United States Department of Agriculture, Agricultural Research Service, Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center, Hilo, HI 96720, USA
Thanapat Songsak
Department of Pharmacognosy, Faculty of Pharmacy, Rangsit University, Pathumtani 12000, Thailand
Supakit Wongwiwatthananukit
Department of Pharmacy Practice, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, Hilo, HI 96720, USA
Leng Chee Chang
Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawai‘i at Hilo, 34 Rainbow Drive, Hilo, HI 96720, USA

Abstract

Bocconia frutescens is an invasive shrub originally introduced to Hawai’i as an ornamental plant. Gomphocarpus physocarpus is a shrub that is native to southern Africa, also introduced to Hawai’i. These two plants were selected for study due to the possibility of alkaloid contents and biological activities. The objective of this study was to examine the protein kinase inhibitory activity (PKI) of crude methanol extracts derived from Bocconia frutescens and Gomphocarpus physocarpus using hyphae formation inhibition assay against Streptomyces 85E at concentrations of 20 μg/disk. Extracts that showed protein kinase inhibitory  activity at this concentration were tested at lower concentrations. Results found that all B. frutescens extracts showed PKI activity, whereas none of the G. physocarpus showed biological activity. In addition, the presence of chelerythrine and berberine alkaloids in  B. frutescens  extracts were assessed during this study using thin layer chromatography (TLC) and liquid chromatography-massspectrometry (LC-MS) analysis. The results showed that both alkaloids were present in all B. frutescens extracts.

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1.
Thomas K, Zhang M, Reinicke J, Parker J, Ohora D, Wall MM, Songsak T, Wongwiwatthananukit S, Chang LC. PROTEIN KINASE INHIBITORY PROPERTIES OF EXTRACTS DERIVED FROM BOCCONIA FRUTESCENS AND GOMPHOCARPUS PHYSOCARPUS. Interprof J Health Sci [Internet]. 2023 Oct. 5 [cited 2024 Sep. 17];15(1):47-58. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/163
Issue
Vol. 15 No. 1 (2017): January - June
Section
Research Articles
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