DETECTION OF CAMPYLOBACTER SPP. IN FRESH CHICKEN MEAT USING DIFFERENT CULTURING PROTOCOLS
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Abstract
This study aimed to compare different culturing protocols for detecting Campylobacter spp. in fresh chicken meat. We evaluated pre-selective enrichment and direct plating protocols using two agar plate media; modified charcoal cefoperazone deoxycholate agar (mCCDA) and blood agar plate through filter membrane (BAF). In the isolation step, two incubation temperatures (37oC, 42oC) in microaerophilic conditions were assessed. The results revealed that pre-selective enrichment protocol had higher sensitivity than direct plating protocol and the optimum incubation was 42oC. Detection limit of artificially inoculated Campylobacter jejuni reference strain in chicken meat isolated on mCCDA and BFA were 10 and 104 CFU/ml respectively. A total of 115 chicken meat samples collected from fresh markets were investigated for Campylobacter spp. contamination. The overall prevalence of C. jejuni that positive by mCCDA or BAF protocol was 12.17% (14/115). In conclusion, pre-selective enrichment step followed by culturing on mCCDA found to be a good protocol for Campylobacter spp. isolation but high flora bacterial contamination was found. The BAF demonstrated an interesting protocol as it is simple, cost-effective and no flora bacterial contamination. Further improvement to get better sensitivity of BAF will be useful for Campylobacter spp. isolation from fresh chicken meat.
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References
Chon, J.-W., Hyeon, J.-Y., Choi, I.-S., Park, C.-K., Kim, S.-K., Heo, S., … Seo, K.-H. (2011). Comparison of three selective media and validation of the VIDAS Campylobacter assay for the detection of Campylobacter jejuni in ground beef and fresh-cut vegetables. Journal of Food Protection, 74(3), 456–460. https://doi.org/10.4315/0362-028X.JFP-10-302
Chon, J.-W., Hyeon, J.-Y., Park, J.-H., Song, K.-Y., & Seo, K.-H. (2012). Comparison of 2 types of broths and 3 selective agars for the detection of Campylobacter species in whole-chicken carcass-rinse samples. Poultry Science, 91(9), 2382–2385. https://doi.org/10.3382/ps.2011-01980
Goossens, H., Pot, B., Vlaes, L., Van den Borre, C., Van den Abbeele, R., Van Naelten, C., … Verhoef, J. (1990). Characterization and description of “Campylobacter upsaliensis” isolated from human feces. Journal of Clinical Microbiology, 28(5), 1039–1046.
Habib, I., Uyttendaele, M., & De Zutter, L. (2011). Evaluation of ISO 10272:2006 standard versus alternative enrichment and plating combinations for enumeration and detection of Campylobacter in chicken meat. Food Microbiology, 28(6), 1117–1123. https://doi.org/10.1016/j.fm.2011.03.001
Hugas, M., Tsigarida, E., Robinson, T., & Calistri, P. (2009). The EFSA Scientific panel on biological hazards first mandate: May 2003–May 2006. Insight into foodborne zoonoses. Trends in Food Science & Technology, 20(5), 188–193. https://doi.org/10.1016/j.tifs.2009.03.001
Hughes, R. A., & Cornblath, D. R. (2005). Guillain-Barré syndrome. The Lancet, 366(9497), 1653–1666. https://doi.org/10.1016/S0140-6736 (05)67665-9
Kaakoush, N. O., Castaño-Rodríguez, N., Mitchell, H. M., & Man, S. M. (2015). Global epidemiology of Campylobacter Infection. Clinical Microbiology Reviews, 28(3), 687–720. https:// doi.org/ 10.1128/CMR.00006-15
Kamata, K., & Tokuda, Y. (2014). Rapid diagnosis of Campylobacter jejuni by stool gram stain examination. Case Reports, 2014(jan28 1), bcr2013202876-bcr2013202876. https://doi.org/10.1136/bcr-2013-202876
Kiess, A. S., Parker, H. M., & McDaniel, C. D. (2010). Evaluation of different selective media and culturing techniques for the quantification of Campylobacter ssp. from broiler litter. Poultry Science, 89(8), 1755–1762. https://doi.org/10.3382/ps.2009-00587
Kramer, J. M., Frost, J. A., Bolton, F. J., & Wareing, D. R. A. (2000). Campylobacter contamination of raw meat and poultry at retail sale: Identification of multiple types and comparison with isolates from human infection. Journal of Food Protection, 63(12), 1654–1659. https://doi.org/10.4315/0362-028X-63.12.1654
Kulkarni, S. P., Lever, S., Logan, J. M. J., Lawson, A. J., Stanley, J., & Shafi, M. S. (2002). Detection of campylobacter species: a comparison of culture and polymerase chain reaction based methods. Journal of Clinical Pathology, 55(10), 749–753.
Man, S. M. (2011). The clinical importance of emerging Campylobacter species. Nature Reviews Gastroenterology & Hepatology, 8(12), 669–685. https://doi.org/10.1038/nrgastro.2011.191
Meeyam, T., Padungtod, P., & Kaneene, J. B. (2004). Molecular characterization of Campylobacter isolated from chickens and humans in northern Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health, 35(3), 670–675.
Nachamkin, I. (2003). Manual of Clinical Microbiology (8th ed.). Washington, D.C.: ASM Press.
Rosenquist, H., Sommer, H. M., Nielsen, N. L., & Christensen, B. B. (2006). The effect of slaughter operations on the contamination of chicken carcasses with thermotolerant Campylobacter. International Journal of Food Microbiology, 108(2), 226–232. https://doi.org/10.1016/j.ijfoodmicro.2005.12.007
Saiyudthong, S., Phusri, K., & Buates, S. (2015). Rapid detection of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in fresh chicken meat and by-products in Bangkok, Thailand, using modified multiplex PCR. Journal of Food Protection, 78(7), 1363–1369. https://doi.org/10.4315/0362-028X.JFP-14-415
Sasaki, Y., Maruyama, N., Zou, B., Haruna, M., Kusukawa, M., Murakami, M., … Yamada, Y. (2013). Campylobacter cross-contamination of chicken products at an abattoir. Zoonoses and Public Health, 60(2), 134–140. https://doi.org/10.1111/j.1863-2378.2012.01509.x
Skarp, C. P. A., Hänninen, M.-L., & Rautelin, H. I. K. (2016). Campylobacteriosis: the role of poultry meat. Clinical Microbiology and Infection, 22(2), 103–109. https://doi.org/10.1016/j.cmi.2015.11.019
Snelling, W. J., Matsuda, M., Moore, J. E., & Dooley, J. S. G. (2005). Campylobacter jejuni. Letters in Applied Microbiology, 41(4), 297–302. https://doi.org/10.1111/j.1472-765X.2005.01788.x
Steele, T. W., & McDermott, S. N. (1984). The use of membrane filters applied directly to the surface of agar plates for the isolation of Campylobacter jejuni from feces. Pathology, 16(3), 263–265. https://doi. org/ 10.3109/00313028409068535
Ugarte-Ruiz, M., Gómez-Barrero, S., Porrero, M. C., Alvarez, J., García, M., Comerón, M. C., … Domínguez, L. (2012). Evaluation of four protocols for the detection and isolation of thermophilic Campylobacter from different matrices. Journal of Applied Microbiology, 113(1), 200–208. https://doi.org/10.1111/j.1365-2672.2012.05323.x
Vaz, C. S. L., Voss-Rech, D., Pozza, J. S., Coldebella, A., & Silva, V. S. (2014). Isolation of Campylobacter from Brazilian broiler flocks using different culturing procedures. Poultry Science, 93(11), 2887–2892. https://doi.org/10.3382/ps.2014-03943
Vindigni, S. M., Srijan, A., Wongstitwilairoong, B., Marcus, R., Meek, J., Riley, P. L., & Mason, C. (2007a). Prevalence of foodborne microorganisms in retail foods in Thailand. Foodborne Pathogens and Disease, 4(2), 208–215. https://doi.org/10.1089/fpd.2006.0077
Vindigni, S. M., Srijan, A., Wongstitwilairoong, B., Marcus, R., Meek, J., Riley, P. L., & Mason, C. (2007b). Prevalence of foodborne microorganisms in retail foods in Thailand. Foodborne Pathogens and Disease, 4(2), 208–215. https://doi.org/10.1089/fpd.2006.0077
Whyte, P., McGill, K., Cowley, D., Madden, R. ., Moran, L., Scates, P., … Cormican, M. (2004). Occurrence of Campylobacter in retail foods in Ireland. International Journal of Food Microbiology, 95(2), 111–118. https://doi.org/10.1016/j.ijfoodmicro.2003.10.018
Wilson, I. G. (2002). Salmonella and Campylobacter contamination of raw retail chickens from different producers: a six year survey. Epidemiology and Infection, 129(3), 635. https://doi.org/10.1017/S0950268802007665
Wisessombat, S., Kittiniyom, K., Srimanote, P., Wonglumsom, W., & Voravuthikunchai, S. P. (2009). A novel method and simple apparatus for the detection of thermophilic Campylobacter spp. in chicken meat products. Journal of Microbiological Methods, 76(2), 169–173. https://doi.org/10.1016/j.mimet.2008.10.007