INHIBITORY EFFECTS OF GREEN TEA EXTRACT ON ADP AND THROMBIN INDUCED PLATELET AGGREGATION

Article Sidebar

PDF
Published: Oct 5, 2023
Keywords:
Green tea platelet aggregation adenosine diphosphate (ADP) thrombin

Main Article Content

Tipsuchon Aiamsa-ard
Departmant of Pharmacology, Faculty of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand
Watcharaporn Devakul Na Ayutthaya
Departmant of Basic Medical Science, Faculty of Medicine Vajira Hospital, Navamindradhiraj University, Bangkok 10300, Thailand
Thidarat Phetmanee
Departmant of Pharmacology, Faculty of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand

Abstract

The study was performed to investigate the effects of green tea extract (GTE) on platelet aggregation in Wistar rats.  Forty male rats were used in this study and treated with GTE for 35 days. Experimental groups were as follows; control group; distilled water, aspirin 10 mg/kg/day, green tea extract 25, 50 and 100 mg/ kg/day. The platelet aggregation was measured by using microplate reader after inducing platelet aggregation by adenosine diphosphate (ADP) and thrombin. The results showed that green tea extract 25, 50 and 100 mg/kg/day tended to inhibit platelet aggregation induced by ADP and thrombin. However, only green tea 25 mg/kg/day significantly suppressed platelet aggregation when compared with the control group (p<0.05). This experiment showed the potential preventive effects of low dose GTE against platelet aggregation via ADP and thrombin pathway. Thus, green tea extract may be beneficial for preventing against cardiovascular diseases.

Article Details

How to Cite
1.
Aiamsa-ard T, Devakul Na Ayutthaya W, Phetmanee T. INHIBITORY EFFECTS OF GREEN TEA EXTRACT ON ADP AND THROMBIN INDUCED PLATELET AGGREGATION. Interprof J Health Sci [Internet]. 2023 Oct. 5 [cited 2024 Nov. 22];15(1):78-84. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/166
Issue
Vol. 15 No. 1 (2017): January - June
Section
Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Journal of TCI is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence, unless otherwise stated. Please read our Policies page for more information.

References

Deana, R., Turetta, L., Donella-Deana, A., Donà, M., Brunati, A. M., De Michiel, L., & Garbisa, S. (2003). Green tea epigallocatechin-3-gallate inhibits platelet signalling pathways triggered by both proteolytic and non-proteolytic agonists. Thrombosis and haemostasis, 89(5), 866-874.

Durairaj, B., & Dorai, A. (2010). Antiplatelet activity of white and pink Nelumbo nucifera Gaertn flowers. Brazilian Journal of Pharmaceutical Sciences, 46(3), 579-583.

Frei, B., & Higdon, J. V. (2003). Antioxidant activity of tea polyphenols in vivo: evidence from animal studies. The Journal of nutrition, 133(10), 3275S-3284S.

Hertog, M. G. L., Feskens, E. J. M., Kromhout, D., Hertog, M. G. L., Hollman, P. C. H., Hertog, M. G. L., & Katan, M. B. (1993). Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. The Lancet, 342(8878), 1007-1011.

Higdon, J. V., & Frei, B. (2003). Tea Catechins and Polyphenols: Health Effects, Metabolism, and Antioxidant Functions. Critical Reviews in Food Science and Nutrition, 43(1), 89-143.

Kang, W.-S., Lim, I.-H., Yuk, D.-Y., Chung, K.-H., Park, J.-B., Yoo, H.-S., & Yun, Y.-P. (1999). Antithrombotic Activities of Green Tea Catechins and (−)-Epigallocatechin Gallate. Thrombosis Research, 96(3), 229-237.

Khalesi, S., Sun, J., Buys, N., Jamshidi, A., Nikbakht-Nasrabadi, E., & Khosravi-Boroujeni, H. (2014). Green tea catechins and blood pressure: a systematic review and meta-analysis of randomised controlled trials. European journal of nutrition, 53(6), 1299-1311.

Krause, S., Scholz, T., Temmler, U., & Lösche, W. (2001). Monitoring the effects of platelet glycoprotein IIb/IIIa antagonists with a microtiter plate method for detection of platelet aggregation. Platelets, 12(7), 423-430.

Lee, D.-H., Kim, Y.-J., Kim, H.-H., Cho, H.-J., Ryu, J.-H., Rhee, M. H., & Park, H.-J. (2013). Inhibitory effects of epigallocatechin-3-gallate on microsomal cyclooxygenase-1 activity in platelets. Biomolecules and Therapeutics, 21(1), 54-59.

Lill, G., Voit, S., Schrör, K., & Weber, A.-A. (2003). Complex effects of different green tea catechins on human platelets. FEBS letters, 546(2-3), 265-270.

Mosawy, S., Gaiz, A., Karaksha, A., & Singh, I. (2016). The Green Tea Extract Epigallocatechin Gallate Inhibits Human Platelet Function but not Plasma Coagulation. International Journal of Prevention and Treatment, 5(2), 17-21.

Ok, W.-J., Cho, H.-J., Kim, H.-H., Lee, D.-H., Kang, H.-Y., Kwon, H.-W., . . . Park, H.-J. (2012). Epigallocatechin-3-gallate has an anti-platelet effect in a cyclic AMP-dependent manner. Journal of atherosclerosis and thrombosis, 19(4), 337-348.

Packham, M. A., & Mustard, J. F. (1986). The role of platelets in the development and complications of atherosclerosis. Paper presented at the Seminars in hematology.

Ruggeri, Z. (1997). Mechanisms initiating platelet thrombus formation. Thrombosis and haemostasis, 78(1), 611-616.

Sagesaka-Mitane, Y., Miwa, M., & Okada, S. (1990). Platelet aggregation inhibitors in hot water extract of green tea. Chemical and Pharmaceutical Bulletin, 38(3), 790-793.

Weitz, J. I. (2011). Blood Coagulation and Anticoagulant, Fibrinolytic, and Antiplatelet Drugs. In L. L. Brunton, B. A. Chabner, & B. C. Knollmann (Eds.), Goodman & Gilman's: The Pharmacological Basis of Therapeutics, 12e. New York, NY: McGraw-Hill Education.

Yadav, A., & Mendhulkar, V. D. (2015). In vitro anti-platelet aggregation activity of the extracts of Camellia Sinensis. Research in Biotechnology, 6(3), 10-16.