ANTI-INFLAMMATION AND ANTIPLATELET AGGREGATION OF CLOVE OIL IN RATS
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Abstract
Clove (Syzygium aromaticum (L.) Merr.&L.M.Perry) is a herb in the Myrtaceae family. It mainly contains eugenol which is beneficial in treatment of many health conditions including use in dental care as it has antiseptic, analgesic, carminative and antispasmodic effects and it also improves peristalsis. This study aimed to determine the effects of clove oil on acute inflammation, platelet aggregation and hepatic function. Rats were divided into five groups: control, aspirin 150 mg/kgBW/day, clove oil 100, 200 and 400 mg/kgBW/day. Anti-inflammatory effect was studied by using 1% carrageenan-induced rat paw edema test measured by plethysmometer. The platelet aggregation was measured by using microplate reader at 650 nm and adenosine diphosphate (ADP) was used as platelet agonist. Liver function was assessed by measurement of hepatic marker enzymes, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum. The results showed that clove oil at doses of 200 and 400 mg significantly inhibited paw edema more potently than aspirin (p<0.05). In addition, clove oil at the dose of 400 mg/kgBW/day significantly inhibited ADP-induced platelet aggregation (p<0.05). Clove oil did not have an effect on AST and ALT. These observations suggested the possibility role of clove oil in treatment of acute inflammation and prevention of thrombosis without causing hepatotoxicity.
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