BIOLOGICAL PROPERTIES OF AQUEOUS EXTRACT FROM SUREGADA MULTIFLORUM (A. JUSS.) BAILL. STEM AND ANTICANCER RECIPE
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Abstract
uregada multiflorum (A. Juss.) Baill. or Khan-thong-phayabat has been used with other herbs as an anticancer recipe. The aims of this study were to evaluate phytochemicals, the total phenolic content (TPC), antioxidant, antibacterial and cytotoxic activities of aqueous extract of dried stems of S. multiflorum (SM) and anticancer recipe (AR). The phytochemical analysis was performed using the previously described methods. The total phenolic content (TPC) was estimated spectrophotometrically using Folin-Ciocalteu method. Antioxidant activity was determined using DPPH and ABTS assays. The antibacterial activity of the extracts was investigated using the agar disk diffusion method. The diphenyltetrazolium (MTT) assay was used to determine antiproliferative activity. The phytochemical studies of SM extract revealed the presence of flavonoids, alkaloids, glycosides, saponins and phenolics. TPC of AR extract was two times as much of SM extract. The SM and AR extracts exhibited scavenging effects on DPPH and ABTS radicals. However, the AR extract has more total phenolic content and antioxidant activity than SM extract. The SM extract did not exhibit antibacterial activity against all tested bacteria while AR extract showed inhibition effects on the growth of two gram-positive bacterial strains, Staphylococcus aureus and Staphylococcus epidermidis with inhibition zones of 13.57±0.57 mm and 14.57±0.57 mm, respectively. The MIC and MBC values of the AR extract against S. aureus and S. epidermidis were 7.8 mg/mL and 15.62 mg/mL, respectively. Besides that, the MTT assay showed that the SM and AR had a potential antiproliferative activity against human hepatocellular liver carcinoma (HepG2) and human cervical cancer (HeLa) cells in both a dose- and time- dependent manner. Therefore, an investigation on biological activities of aqueous extract of S. multiflorum which has been used in anticancer recipe revealed that the aqueous extract of the stem would contribute to promoting cancer treatment as shown by antioxidant activity and inhibition activity on carcinoma cell viability. It was concluded that the Thai medicinal plant recipe gave antioxidant, antibacterial and anticancer activities.
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