APPLICATION OF EXPRESSED RECOMBINANT OUTER MEMBRANE PROTEIN (OMPA PROTEIN) FROM BURKHOLDERIA THAILANDENSIS E264 IN DOT BLOT ELISA

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Published: Oct 5, 2023
Keywords:
OmpA protein Burkholderia thailandensis Dot blot ELISA Melioidosis

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Kanwitchayut Sroiphetcharat
Biomedical Sciences Graduate Program, Department of Medical Science, Faculty of Science, Rangsit University, Pathumthani 12000, Thailand
Sumet Wajanarogana
Department of Basic Medical Science, Faculty of Medicine Vjira Hospital, Navamindradhiraj University, Bangkok 10300, Thailand
Kanyanan Kritsiriwuthinan
Faculty of Medical Technology, Rangsit University, Pathumtani 12000, Thailand
Patamaporn Sukplang
Microbiology unit, Department of Medical Sciences, Faculty of Science, Rangsit University, Pathumtani 12000, Thailand
Acharawan Thongmee
Microbiology unit, Department of Medical Sciences, Faculty of Science, Rangsit University, Pathumtani 12000, Thailand

Abstract

The recombinant OmpA protein from Burkholderia thailandensis, the closely linked species to Burkholderia pseudomallei, was expressed in E. coli BL21 (DE3).  The purified OmpA protein was assessed as an antigen that specifically reacts to melioidosis antibodies by dot blot ELISA.  In this study implementations were practiced with 5 bacterial-culture-confirmed melioidosis sera and 3 healthy donor sera.  At the serum dilutions of 1:800, 1:1,600 and 1:3,200, the sensitivities of 100%, 100%, 60% and specificities of 0%, 0%, 100% were shown, respectively.  Therefore, it can be concluded that the B. thailandensis OmpA protein antigen developed may possibly be used for a serological diagnosis of melioidosis by dot blot ELISA at the cut-off titer of 3,200 with sensitivity and specificity of 60% and 100%.

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How to Cite
1.
Sroiphetcharat K, Wajanarogana S, Kritsiriwuthinan K, Sukplang P, Thongmee A. APPLICATION OF EXPRESSED RECOMBINANT OUTER MEMBRANE PROTEIN (OMPA PROTEIN) FROM BURKHOLDERIA THAILANDENSIS E264 IN DOT BLOT ELISA. Interprof J Health Sci [Internet]. 2023 Oct. 5 [cited 2024 Nov. 22];15(2):54-62. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/174
Issue
Vol. 15 No. 2 (2017): July - December
Section
Research Articles
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