ESTIMATION OF CENTELLOIDS AND TOTAL PHENOLIC CONTENT OF CENTELLA ASIATICA (L.) URB. USING COMPUTER SOFTWARE AND THEIR CORRELATION WITH ANTIOXIDANT ACTIVITY
Article Sidebar
Main Article Content
Abstract
Centella asiatica (L.) Urb. is a herbal plant with wound healing property and several biological activities. The aims of this work were to estimate the centelloids and total phenolic content of Centella asiatica (L.) Urb. using computer software and investigate the linear correlation between centelloids or total phenolic content and the antioxidant activity. The two-factor spherical composite experimental design was performed to estimate the content of centelloids and total phenolic compound. Extraction temperature and extraction time were varied five levels from 40 to 60 °C and 60 to 120 min, respectively. The increment of temperature and time provided a high content of centelloids. The higher content of total phenolic compound was achieved at the high temperature and medium extraction time. The estimation equation of both two groups of compound fitted with quadratic model. The estimation was reliable and stable. The optimal condition provided the highest centelloids content was 59 °C and 108.1 min. While 55.7 °C and 77.4 min gave the highest total phenolic content. The correlation study using statistical software between centelloids or total phenolic content and antioxidant activity was performed, centelloids content had a negative effect on antioxidant activity from 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. But, no correlation was found from ferric ion reducing antioxidant power (FRAP) assay. Total phenolic content did not correlate with antioxidant activity from DPPH radical scavenging assay as well as FRAP assay. These results are valuable for our future work as a standard condition for extraction of Centella asiatica (L.) Urb. to obtain the high content of active chemical constituent.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Journal of TCI is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence, unless otherwise stated. Please read our Policies page for more information.
References
Benzie IFF, Strain JJ. 1996. The Ferric Reducing Ability of Plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry. 239(1): 70-76.
Bylka W, Znajdek-Awiżeń P, Studzińska-Sroka E, Dańczak-Pazdrowska A, Brzezińska M. 2014. Centella asiatica in dermatology: An overview. Phytotherapy Research. 28(8): 1117-1124.
Chew KK, Ng SY, Thoo YY, Khoo MZ, Wan Aida WM, Ho CW. 2011. Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Centella asiatica extracts. International Food Research Journal. 18: 571-578.
Dent M, Dragović-Uzelac V, Penić M, Brnčić M. 2013. The effect of extraction solvents, temperature and time on the composition and mass fraction of polyphenols in Dalmatian wild sage (Salvia officinalis L.) extracts. Food Technology and Biotechnology. 51(1): 84-91.
Duangjit S, Mehr LM, Kumpugdee-Vollrath M, Ngawhirunpat T. 2014. Role of simplex lattice statistical design in the formulation and optimization of microemulsions for transdermal delivery. Biological & Pharmaceutical Bulletin. 37(12): 1948-1957.
Giada MdLR. 2013. Food phenolic compounds: Main classes, sources and their antioxidant power. In J. A. Morales-González (Ed.), Oxidative stress and chronic degenerative diseases - A role for antioxidants. pp. 87-112. Rijeka: InTech.
Gibson M. 2016. Pharmaceutical preformulation and formulation: A practical guide from candidate drug selection to commercial dosage form. 2nd ed. Vol. 199. New York: Informa Healthcare USA, Inc.
Hou W, Zhang W, Chen G, Luo Y. 2016. Optimization of extraction conditions for maximal phenolic, flavonoid and antioxidant activity from Melaleuca bracteata leaves using the response surface methodology. PLOS ONE. 1-16.
Khare CP. 2007. Centella asiatica (Linn.) Urban. Indian medicinal plants: An illustrated dictionary. pp. 1. New York: Springer New York.
Liu M, Dai Y, Li Y, Luo Y, Huang F, Gong Z, Meng Q. 2008. Madecassoside isolated from Centella asiatica herbs facilitates burn wound healing in mice. Planta Medica. 74(8): 809-815.
Liu Y-K, Yan E, Zhan H-Y, Zhang Z-Q. 2011. Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii. Journal of Pharmaceutical Analysis. 1(1): 13-19.
Mat Nor NA, Arof AK. 2016. Optimization of extraction parameters for anthocyanin from Ixora siamensis by response surface methodology. Optical and Quantum Electronics. 48(3): 204.
Pan Y, Zhang J, Shen T, Zuo Z-T, Jin H, Wang Y-Z, Li W-Y. 2015. Optimization of ultrasonic extraction by response surface methodology combined with ultrafast liquid chromatography–ultraviolet method for determination of four iridoids in Gentiana rigescens. Journal of Food and Drug Analysis. 23(3): 529-537.
Pandey KB, Rizvi SI. 2009. Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Medicine and Cellular Longevity. 2(5): 270-278.
Rice-Evans C, Miller N, Paganga G. 1997. Antioxidant properties of phenolic compounds. Trends in Plant Science. 2(4): 152-159.
Shukla A, Rasik AM, Dhawan BN. 1999. Asiaticoside-induced elevation of antioxidant levels in healing wounds. Phytotherapy Research. 13(1): 50-54.
Shukla A, Rasik AM, Jain GK, Shankar R, Kulshrestha DK, Dhawan BN. 1999. In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica. Journal of Ethnopharmacology. 65(1): 1-11.
Slinkard K, Singleton VL. 1997. Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture. 28: 49-55.
Vassallo N (Ed.). (2008). Polyphenols and health: New and recent advances. New York: Nova Science Publishers.
Vergara-Salinas JR, Perez-Jimenez J, Torres JL, Agosin E, Perez-Correa JR. 2012. Effects of temperature and time on polyphenolic content and antioxidant activity in the pressurized hot water extraction of deodorized thyme (Thymus vulgaris). Journal of Agricultural and Food Chemistry. 60(44): 10920-10929.
Wong SP, Leong LP, William Koh JH. 2006. Antioxidant activities of aqueous extracts of selected plants. Food Chemistry. 99(4): 775-783.
Yim HS, Chye FY, Rao V, Low JY, Matanjun P, How SE, Ho CW. 2013. Optimization of extraction time and temperature on antioxidant activity of Schizophyllum commune aqueous extract using response surface methodology. Journal of Food Science and Technology. 50(2): 275-283.
Yuan X, Zeng Y, Nie K, Luo D, Wang Z. 2015. Extraction optimization, characterization and bioactivities of a major polysaccharide from Sargassum thunbergii. PLOS ONE. 1-11.