TLC Densitometry Analysis of Cannabis Flower Growing in Thailand
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Abstract
The inflorescence cannabis of Hang Kra Rog Phu Phan (Cannabis sativa L.) has attracted interests as a raw plant material due to its high tetrahydrocannabinol (THC) content. This study aimed to investigate the THC and cannabidiol (CBD) contents of inflorescence cannabis samples from different regions in Thailand. TLC densitometry determined the contents of THC and CBD on silica gel G60 F254 and hexane-dichloromethane (1:1) as a mobile phase. This method was validated for THC analysis and TLC method resulted in linear regression with the calibration curve of 300-900 ng for THC. The THC and CBD contents were in the range of 7.29 – 12.70 %w/w and 0.11 – 0.73 %w/w, respectively. Prior to the analysis, the decarboxylation reaction by heating in a hot air oven was a favorable sample preparation to maximize the THC and CBD contents. According to the Notification of the Department of Agriculture the Plant Varieties Act reported equal amounts of THC and CBD of this breed. However, all samples showed relatively high THC contents comparing to CBD contents. The highest THC content (12.70 %w/w) was from Nakhon Phanom 2.
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References
Department of Agriculture. The publish of letter of recommendation of registered plant breed: Plant Varieties Act. 2021.
Department of Medical Sciences. Supplement 2022 Kancha, Bai. Thai Herbal Pharmacopoeia: Bureau of Drug and Narcotic, Department of medical Sciences, Ministry of Public Health; 2021.
Debruyne D, Albessard F, Bigot MC, Moulin M. Comparison of three advanced chromatographic techniques for cannabis identification. Bull Narc. 1994;46(2):109-21.
Wang M, Wang YH, Avula B, Radwan MM, Wanas AS, van Antwerp J, et al. Decarboxylation Study of Acidic Cannabinoids: A Novel Approach Using Ultra-High-Performance Supercritical Fluid Chromatography/Photodiode Array-Mass Spectrometry. Cannabis Cannabinoid Res. 2016;1(1):262-71. https://doi.org/10.1089/can.2016.0020
Sherma J, Rabel F. Thin layer chromatography in the analysis of cannabis and its components and synthetic cannabinoids. J Liq Chromatogr Relat. 2019;42(19-20):613-28. https://doi.org/10.1080/10826076.2019.1663529
Liu Y, Brettell TA, Victoria J, Wood MR, Staretz ME. High performance thin-layer chromatography (HPTLC) analysis of cannabinoids in cannabis extracts. Forensic Chem. 2020;19:100249. https://doi.org/10.1016/j.forc.2020.100249
The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH). ICH-Guidelines Q2(R1), Validation of Analytical Procedures: Text and Methodology 2005 [3 December 2022]. Available from: https://database.ich.org/sites/default/files/Q2%28R1%29%20Guideline.pdf.
AOAC Official Methods of Analysis. Appendix F. Guidelines for standard method performance requirements. MD, USA.: AOAC International Rockville; 2016.
Department of Medical Sciences. Kancha, Ruean cho dok phet mia. Thai Herbal Pharmacopoeia: Bureau of Drug and Narcotic, Department of Medical Sciences, Ministry of Public Health; 2021. p. 153-68.
Fischedick JT, Glas R, Hazekamp A, Verpoorte R. A qualitative and quantitative HPTLC densitometry method for the analysis of cannabinoids in Cannabis sativa L. Phytochem Anal. 2009;20(5):421-6. https://doi.org/10.1002/pca.1143
Alexander. Beadle. Scientists pinpoint ideal decarboxylation condition to maximize THC and CBD: Analytical Cannabise; 2020 [3 December 2022]. Available from: https://www.analyticalcannabis.com/news/scientists-pinpoint-ideal-decarboxylation-conditions-to-maximize-thc-and-cbd-312843.
Mohammad A, Sharma S, Bhawani S, Singh R. Identification and separation of Cannabis sativa, Embleia ribes, Myristica fragrans and Piper longum from organic extract on silica gel surface with anionic micellar solvent system: application in ayurvedic medicine. Open Nutraceuticals J. 2010;3:112-8. https://doi.org/10.2174/18763960010030300112
Department of Medical Sciences. Supplement 2023 Kancha, Rak. Thai Herbal Pharmacopoeia: Bureau of Drug and Narcotic, Department of Medical Sciences, Ministry of Public Health; 2021. p. 24-9.
Thongchin T, Marsud S, Thiemthieprat P, Ruengkhet S, Ontong S, Banyati P, et al. Development and method validation of cannabinoid contents in Cannabis sativa L. leaves by ultra high-performance liquid chromatography. Bull Dept Med Sci. 2021;63(3):505-23.
Ross SA, Elsohly MA. CBN and Delta 9-THC concentration ratio as an indicator of the age of stored marijuana samples. Bull Narc. 1997;49:139-47. https://doi.org/10.18356/2ba05fac-en