TLC Densitometry Analysis of Cannabis Flower Growing in Thailand

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Laksana Charoenchai
Chaowalit Monton
Thaniya Wunnakup
Famera Madaka
Artimond Vutthipong
Thanapat Songsak

Abstract

The inflorescence cannabis of Hang Kra Rog Phu Phan (Cannabis sativa L.) has attracted interests as a raw plant material due to its high tetrahydrocannabinol (THC) content. This study aimed to investigate the THC and cannabidiol (CBD) contents of inflorescence cannabis samples from different regions in Thailand. TLC densitometry determined the contents of THC and CBD on silica gel G60 F254 and hexane-dichloromethane (1:1) as a mobile phase. This method was validated for THC analysis and TLC method resulted in linear regression with the calibration curve of 300-900 ng for THC. The THC and CBD contents were in the range of 7.29 – 12.70 %w/w and 0.11 – 0.73 %w/w, respectively. Prior to the analysis, the decarboxylation reaction by heating in a hot air oven was a favorable sample preparation to maximize the THC and CBD contents. According to the Notification of the Department of Agriculture the Plant Varieties Act reported equal amounts of THC and CBD of this breed. However, all samples showed relatively high THC contents comparing to CBD contents. The highest THC content (12.70 %w/w) was from Nakhon Phanom 2.

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How to Cite
1.
Charoenchai L, Monton C, Wunnakup T, Madaka F, Vutthipong A, Songsak T. TLC Densitometry Analysis of Cannabis Flower Growing in Thailand. Interprof J Health Sci [Internet]. 2023 Dec. 23 [cited 2024 Dec. 6];21(1-2):IJHS-0256. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/256
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Research Articles

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