ANTIMICROBIAL EFFICACY OF CONTACT LENS SOLUTIONS AGAINST PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS
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Abstract
Contact lenses are used as an alternative to eyeglasses for correction of eyesight problems. However, contact lenses wearers are at risk for bacterial keratitis. To minimize the risk of eye infections, contact lens care solutions were used in order to prevent potential pathogens during contact lens storage. This study aimed to evaluate the antibacterial activity of multipurpose contact lens care solutions available in Thailand against the common causes of bacterial keratitis, Pseudomonas aeruginosa and Staphylococcus aureus. Four multipurpose contact lens care solutions, designated as A, B, C and D, were determined for antimicrobial activities against methicillin-resistant Staphylococcus aureus (MRSA) ATCC43300, methicillin-sensitive Staphylococcus aureus (MSSA) ATCC25930 and Pseudomonas aeruginosa ATCC27853 by agar-well diffusion method. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were also determined by broth microdilution assay. In addition, the contact time between test bacteria and each contact lens care solution was determined for the effective duration time that can reduce 99.9% (3logs) of test bacteria. The results showed that all test contact lens care solutions did not show inhibition zone against test bacteria by agar-well diffusion method. However, contact lens care solution A at the concentration ranging between 12.5-25% of the initial concentration inhibited the growth of all tested bacteria. In addition, contact lens care solution D has more killing effect than other contact lens care solutions since it can reduce the number of test bacteria more than 99.9% (3logs) in 2 hours. The different in killing effect might be due to the difference in active ingredients in each solutions.
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