IN VITRO ANTI-OXIDANT, ANTI-INFLAMMATORY AND ANTI-MICROBIAL PROPERTIES OF LOCAL HERBAL RECIPE FOR DIABETIC ULCER TREATMENT

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Jintana Junlatat
Kanyarat Peng-ngummuang
Musikorn Tuseewan
Piyaporn Thorajake
Papassorn Opap
Sirilak Kumpapong
Siriporn Konkhayan
Niramai Fangkrathok

Abstract

This study aimed to investigate the antioxidant, anti-inflammatory and anti-microbial properties of the diabetic ulcer-treatment recipe of folk healer, Mr. Kitti Nakhun. For preparation, the recipe was extracted by maceration with 80% ethanol and boiling with water then both extracts were freeze-dried to give 80% ethanol extract (KE) and water extract (KW). Then the extracts were tested for antioxidant and anti-inflammatory activities. The results showed that the KE and KW exhibited antioxidant activity by using DPPH assay (IC50 were 18.66 ± 1.20 and 58.04 ± 8.12 µg/mL, respectively) and the antioxidant activity was extremely related to phenolic content (r2 was 0.99). In addition, the extracts were cytotoxic tested in RAW264.7 macrophage cells by using MTT assay (IC50 of KE and KW were 470 ± 3.54 and 730 ± 3.28 µg/mL, respectively) and examined the anti-inflammatory effect by measuring nitric oxide (NO) production in the cells using Griess reagent. The results showed that after 24 hr of incubation, the 100 µg/mL concentration of KE and KW inhibited the NO production with 43.89 ± 2.72% and 12.95 ± 1.50%, respectively. Moreover, both of the extracts (400 µg/mL, 24 hr of incubation) revealed anti-microbial activity which can be seen from the inhibition zone on gram positive bacteria, Bacillus subtilis and an opportunistic pathogenic yeast, Candida albicans. The results support the traditional use of this recipe for treating diabetic ulcer and are particularly useful for product development.

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How to Cite
1.
Junlatat J, Peng-ngummuang K, Tuseewan M, Thorajake P, Opap P, Kumpapong S, Konkhayan S, Fangkrathok N. IN VITRO ANTI-OXIDANT, ANTI-INFLAMMATORY AND ANTI-MICROBIAL PROPERTIES OF LOCAL HERBAL RECIPE FOR DIABETIC ULCER TREATMENT. Interprof J Health Sci [Internet]. 2020 Oct. 16 [cited 2024 Dec. 6];18(2):44-50. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/36
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Research Articles

References

Abou-Hany HO, Atef H, Said E, Elkashef HA, Salem HA. 2018. Crocin mediated amelioration of oxidative burden and inflammatory cascade suppresses diabetic nephropathy progression in diabetic rats. Chem. Biol. Interact. 284: 90-100.

Ahmad I, Beg ZA. 2001. Antimicrobial and phytochemical studies on 15 Indian medicinal plants against multidrug-resistant human pathogens. J. Ethnopharmacol. 74(2): 113-23.

Bolajoko ES, Moropane M, Adeniyi F, Fasanmade A. 2008. Antioxidant and oxidative stress status in type2 diabetes and diabetic foot ulcer. S. Afr. Med. J. 98(8): 614-7.

Choi SE, Park KH, Han BH, Jeong MS, Seo SJ, Seo SJ, et al. 2011. Inhibitory of inducible nitric oxide synthase and cyclooxygenase-2 expression by phenolic compounds from roots of Rhododendron mucronulatum. Phytother. Res. 25: 1301-5.

Chuangchot C, Tattawasart U, Sripanidkulchai B, Junlatat J, Fangkrathok N. 2017. Antibacterial and antioxidant activity of Rafflesia kerrii extract against multidrug-resistant bacteria. Songklanakarin J. Sci. Technol. 39(2): 163-70.

Conforti F, Menichini F. 2011. Phenolic compounds from plants as nitric oxide production inhibitors. Curr. Med. Chem. 18(8): 1137-45.

Cushnie TP, Andrew AL. 2005. Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents. 26(5): 343-56.

Daglia M. 2012. Polyphenols as antimicrobial agents. Curr. Opin. Biotechnol. 23(2): 174-81.

Fu Y, Xie B, Ben D, Lv K, Zhu S, Lu W, et al. 2012. Pathogenic alteration in severe burn wounds. Burns. 38(1): 90-94.

Karunakaran T, Ismail IS, Ee GCL, Nor SMM, Palachandran KP, Santhanam RK. 2018. Nitric oxide inhibitory and anti-Bacillus activity of phenolic compounds and plant extracts from Mesua species. Braz. J. Pharmacogn. 28: 231-4.

Lee SC, Pervaiz S. 2007. Apoptosis in the pathophysiology of diabetes mellitus. Int. J. Biochem. Cell Biol. 39: 497-504.

Mosmann T. 1983. Rapaid colorimetric assay for cellular growth and survival: applicication to proliferation and cytotoxicity assays. J. Immunol. Methods. 65(1-2): 55-63.

Ni Z, Guo L, Liu F, Olatunji OJ, Yin M. 2019. Allium tuberosum alleviates diabetic nephropathy by suppressing hyperglycemia-induced oxidative stress and inflammation in high fat diet/streptozotocin treated rats. Biomed. Pharmacother. 112: 108678.

Nualkaew S, Rattanamanee K, Thongpraditchote S, Wongkrajang Y, Nahrstedt A. 2009. Anti-inflammatory, analgesic and wound healing activities of the leaves of Memecylon edule Roxb. J. Ethnopharmacol. 121: 278-81.

Ogba OM, Nsan E, Eyam ES. 2019. Aerobic bacteria associated with diabetic foot ulcers and their susceptibility pattern. Biomed. Dermatol. 3(1): 1-6.

Reddy CVK, Sreeramulu D, Raghunath M. 2010. Antioxidant activity of fresh and dry fruits commonly consumed in India. Food Res. Int. 43:285-8.

Roy K, Sedhain A, Ali S. 2015. Medicinal plants: current advancement and approach in the therapy of diabetes mellitus. Univers. J. Pharm. Sci. Res. 1: 20–31.

Sarawong C, Schoenlechner R, Sekiguchi K, Berghofer E, Ng PKW. 2014. Effect of extrusion cooking on the physicochemical properties, resistant starch, phenolic content and antioxidant capacities of green banana flour. Food Chem. 143:33-9.

Sarkar S, Chaudhary A, Saha TK, Das AK. 2018. Modulation of collagen population under honey assisted wound healing in diabetic rat model. Wound Med. 20: 7-17.

Shimada K, Fujikawa K, Yahara K, Nakamura T. 1992. Antixidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J. Agric. Food Chem. 40(6): 945-8.

Snyder RJ, Hanft JR. 2009. Diabetic foot ulcers, effects on QOL, costs, and mortality and the role of standard wound care and advanced-care therapies. Ostomy Wound Manag. 55(11): 28-38.

Srinivasan R, Natarajan D, Shivakumar MS. 2017. Spectral characterization and antibacterial activity of an isolated compound from Memecylon edule leaves. J. Photochem. Photobiol. B: Biol. 168: 20-4.

Sripanidkulchai B, Fangkrathok N. 2014. Antioxidant, antimutagenic and antibacterial activities of extracts from Phyllanthus emblica branches. Songklanakarin J. Sci. Technol. 36(6): 669-74.

Yang EJ, Yim EY, Song G, Kim GO, Hyun CG. 2009. Inhibition of nitric oxide production in lipopolysaccharide-activated RAW264.7 macrophages by Jeju plant extracts. Interdiscip. Toxicol. 2(4): 245-9.