Comparative Evaluation Of RP-HPLC And TLC-Densitometric Method For Determination Of Barakol Content in Senna Siamea Leaves

Abstract

Zingiber cassumunar Roxb. (Plai) is a medicinal plant widely cultivated in Thailand and tropical Asia for Thai traditional fields. The rhizome of Zingiber cassumunar Roxb. has an activity that helps reduce inflammation. It is more frequently used as an ingredient in marketed phytomedicines. This review aimed firstly to present the extraction method of Zingiber cassumunar Roxb. rhizome, using different organic solvents, such as chloroform, hexane, and methanol that obtain the mainly various phenylbutanoid derivatives such as ((E)-4-(3,4-dimethoxyphenyl)but-3-en-l-ol (compound I),  (E)-4-(3, 4-dimethoxyphenyl)but-3-en-l-yl acetate (compound II), (E)-4-(3,4-dimethoxyphenyl) but-3-en-1-O--D-glucopyranoside (compound III), (E)-1-(3, 4-dimethoxyphenyl)butadiene (compound IV), (E)-4-(2,4,5-trimethoxyphenyl)-but-1,3-diene (compound V), (E)-trans-3-(3,4-dimethoxyphenyl)-4-[(E )-3,4-dimethoxystyryl] cyclohex-1-ene (compound VI), and (E)-trans-3-(4-hydroxy-3-methoxyphenyl)-4-[(E)-3,4-dimethoxystyryl]cyclohex-1-ene (compound VII). It has been specially reported that the anti-inflammatory active constituents are compound I, II, V, and VI. The second aim was to suggest the preparation of Zingiber cassumunar Roxb. in new formulations such as emulsions and gels. The oil extract from Zingiber cassumunar Roxb. can be appropriately prepared for further applications in cosmetic or medical applications.