PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF “UM-MA-RUEK-KA-VA-TEE” HERBAL FORMULA

Main Article Content

Saran Chaweerak
Tanit Padumanonda
Prathan Luecha

Abstract

This research aims to study the phytochemical screening and antioxidant activity of “UM-MA-RUEK-KA-VA-TEE” (UM) herbal formula used as antitussive and expectorant. The ingredients in UM including myrobalans have been reported for their high amounts of total phenolic compounds that might exhibit a potential benefit as a natural antioxidant. Phytochemical screening of UM was carried out using standard methods of precipitation and coloration reactions. The samples used in this study are 8 products from different manufacturer sources as well as the individual herb extracts. Analysis of antioxidant activity by 1,1-Diphenyl picryl hydrazyl (DPPH) radical assay was reported as IC50. According to the result, Phyllanthus emblica extract showed the best antioxidant activity (254.76±0.08 µg/ml).  Analysis of antioxidant activity by ferric reducing antioxidant potential (FRAP) assay calculated the ability of FRAP value expressed as mg of Trolox equivalent (TE) per gram crude extract. Samples 3, 7 and 5 had the best antioxidant activity; 242±0.14, 237±0.32 and 216±0.08 mg TE/g crude extract, respectively. Total phenolic content was determined using the standard curve of gallic acid (100-1000 µg/ml). It was found that sample 6 had the highest phenolic content (166 ± 0.06 mg of GA/g of crude extract). Basic phytochemical tests found that 6 samples contained anthraquinones, triterpenoids and tannins. The results from this study will provide the guidelines for the further research and development of UM herbal formula in the future.

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1.
Chaweerak S, Padumanonda T, Luecha P. PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF “UM-MA-RUEK-KA-VA-TEE” HERBAL FORMULA. Interprof J Health Sci [Internet]. 2021 Mar. 2 [cited 2024 Dec. 6];19(1):16-24. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/43
Section
Research Articles

References

Aruoma OI. 1994. Nutrition and health aspects of free radicals and antioxidants. Food Chem. Toxicol. 32(12): 671–83.

Biradar YS, Singh R, Sharma K, Dhalwal K, Bodhankar SL, Khandelwal KR. 2007. Evaluation of anti-diarrhoeal property and acute toxicity of Triphala Mashi, an Ayurvedic formulation. J. Herb. Pharmacother. 7(3-4): 203-12.

Cheeseman KH, Slater TF. 1993. An introduction to free radicals chemistry. Br. Med. Bull. 49(3): 481–93.

Debelle FD, Vanherweghem JL, Nortier JL. 2008. Aristolochic acid nephropathy: a worldwide problem. Elsevier Inc. 74(2):158-69.

Deepa B, Prema G, Sai KB, Cherian KM. 2013. Antioxidant and free radical scavenging activity of triphala determined by using different in vitro models. J. Med. Plant Res. 7(39): 2898-905.

Jagetia GC, Baliga MS, Malagi KJ, Sethukumar KM. 2002. The evaluation of the radioprotective effect of Triphala (an ayurvedic rejuvenating drug) in the mice exposed to gamma-radiation. Phytomedicine 9: 99-108.

Jatav VS, Singh SK, Sharma AK. 2011. Recent Pharmacological trends in Glycyrrhiza glabra. Int. J. Pharm. Front. Res. 1: 170-85

Jirum J, Sangdee A, Srihanam P. 2013. Phytochemical and biological activities in fresh juice extracts of wild grape (Ampelocissus martiniPlanch.) fruits. Int. j. Res. Ayur. Pharm. 4(3), 337-41.

Nawwar MAM, Barakat HH, Buddrust J, and Linscheidt M. 1985. Alkaloidal, lignan and phenolic constituents of Ephedra alata. Phytochemistry 24(4): 878–9.

Ngo LT, Okogun JI, Folk WR. 2013. 21st Century natural product research and drug development and traditional medicines. Nat. Prod. Rep. 30: 584–92.

Parveen R, Shamsi TN, Singh G, Athar T, Fatima S. 2018. Phytochemical analysis and in-vitro biochemical characterization of aqueous and methanolic extract of Triphala, a conventional herbal remedy. Biotechnol. Rep. 17:126–36.

Pastorino G, Cornara L, Soares S, Rodrigues F, Oliveira MBPP. 2018. Liquorice (Glycyrrhiza glabra): A phytochemical and pharmacological review. Phytother. Res. 32(12):2323-39.

Peterson CT, Denniston K, Chopra D. 2017. Therapeutic Uses of Triphala in Ayurvedic Medicine. J. Altern. Complement. Med. 23(8): 607-14.

Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods Enzymol. 299:152-78.

Sorsavanh S, Ruengsitagoon W, Padumanonda T. 2019 Phytochemical screening, total phenolic contents and antioxidant capacities of selected blood tonic formula from Vientiane province, Lao PDR. Isan J. Pharm. Sci. 15(1):181-94.