PHARMACOGNOSTIC SPECIFICATION OF MENTHA CORDIFOLIA LEAF AND STEM WITH SPECIAL REFERENCE TO ROSMARINIC ACID CONTENTS

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Published: Apr 30, 2021
Keywords:
Mentha cordifolia Marsh mint Rosmarinic acid RP-HPLC Crude drug Quality control

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Ploychat Chamnanthongpiwan
Public Health Sciences Program, College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
Chanida Palanuvej
Department of Pharmacognosy, College of Pharmacy, Rungsit University, Pathum Thani, 12000, Thailand
Nijsiri Ruangrungsi
Department of Pharmacognosy, College of Pharmacy, Rungsit University, Pathum Thani, 12000, Thailand

Abstract

Mentha cordifolia Opiz ex Fresen.(Sa-ra-nae) is one of the popular Thai kitchen herbs. This study aimed to specify the quality parameters of M. cordifolia dried leaves and stems with special reference to rosmarinic acid (RA) contents. The results showed that diacytic stomata type were found in upper and lower sides of leaf. The microscopic leaf constant numbers were found to be stomatal index, trichome index, oil gland index and epidermal cell area of upper leaf was 2.85 ± 0.64, 0.45 ± 0.44, 3.09 ± 0.89 and 2128.64 ± 182.80 µm2; of lower leaf was 19.95 ± 2.65, 0.46 ± 0.33, 5.97 ± 1.75 and 1793.52 ±262.45 µm2 respectively. The physicochemical specification demonstrated the contents of acid-insoluble ash, total ash, loss on drying and moisture in leaf should not be more than 1.98, 9.41, 7.06 and 10.50; in stem should not be more than 1.63, 8.40, 7.04, 10.01 % by weight respectively; the ethanol-soluble extractive matter, water-soluble extractive matter and volatile oil in leaf should not be less than 5.04, 15.23 and 0.36; in stem should not be less than 5.20 and 16.60 % by weight respectively. The main chemical compound in the volatile oil was piperitenone oxide (73.22%). RA quantitative analysis was made by RP-HPLC using intentsil® ODS-3 column as stationary phase and methanol: 0.2% phosphoric acid (45% : 55%) as mobile phase. RP-HPLC analysis revealed the RA content in leaf as 1.92 ± 1.27 and in stem as 0.99 ± 0.41 g/100 g by dry weight. 

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1.
Chamnanthongpiwan P, Palanuvej C, Ruangrungsi N. PHARMACOGNOSTIC SPECIFICATION OF MENTHA CORDIFOLIA LEAF AND STEM WITH SPECIAL REFERENCE TO ROSMARINIC ACID CONTENTS. Interprof J Health Sci [Internet]. 2021 Apr. 30 [cited 2024 Nov. 21];19(1):33-44. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/45
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Vol. 19 No. 1 (2021): January - June
Section
Research Articles
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