PHARMACOGNOSTIC SPECIFICATION OF MENTHA CORDIFOLIA LEAF AND STEM WITH SPECIAL REFERENCE TO ROSMARINIC ACID CONTENTS

Article Sidebar

PDF
Published: Apr 30, 2021
Keywords:
Mentha cordifolia Marsh mint Rosmarinic acid RP-HPLC Crude drug Quality control

Main Article Content

Ploychat Chamnanthongpiwan
Public Health Sciences Program, College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
Chanida Palanuvej
Department of Pharmacognosy, College of Pharmacy, Rungsit University, Pathum Thani, 12000, Thailand
Nijsiri Ruangrungsi
Department of Pharmacognosy, College of Pharmacy, Rungsit University, Pathum Thani, 12000, Thailand

Abstract

Mentha cordifolia Opiz ex Fresen.(Sa-ra-nae) is one of the popular Thai kitchen herbs. This study aimed to specify the quality parameters of M. cordifolia dried leaves and stems with special reference to rosmarinic acid (RA) contents. The results showed that diacytic stomata type were found in upper and lower sides of leaf. The microscopic leaf constant numbers were found to be stomatal index, trichome index, oil gland index and epidermal cell area of upper leaf was 2.85 ± 0.64, 0.45 ± 0.44, 3.09 ± 0.89 and 2128.64 ± 182.80 µm2; of lower leaf was 19.95 ± 2.65, 0.46 ± 0.33, 5.97 ± 1.75 and 1793.52 ±262.45 µm2 respectively. The physicochemical specification demonstrated the contents of acid-insoluble ash, total ash, loss on drying and moisture in leaf should not be more than 1.98, 9.41, 7.06 and 10.50; in stem should not be more than 1.63, 8.40, 7.04, 10.01 % by weight respectively; the ethanol-soluble extractive matter, water-soluble extractive matter and volatile oil in leaf should not be less than 5.04, 15.23 and 0.36; in stem should not be less than 5.20 and 16.60 % by weight respectively. The main chemical compound in the volatile oil was piperitenone oxide (73.22%). RA quantitative analysis was made by RP-HPLC using intentsil® ODS-3 column as stationary phase and methanol: 0.2% phosphoric acid (45% : 55%) as mobile phase. RP-HPLC analysis revealed the RA content in leaf as 1.92 ± 1.27 and in stem as 0.99 ± 0.41 g/100 g by dry weight. 

Article Details

How to Cite
1.
Chamnanthongpiwan P, Palanuvej C, Ruangrungsi N. PHARMACOGNOSTIC SPECIFICATION OF MENTHA CORDIFOLIA LEAF AND STEM WITH SPECIAL REFERENCE TO ROSMARINIC ACID CONTENTS. Interprof J Health Sci [Internet]. 2021 Apr. 30 [cited 2024 Sep. 17];19(1):33-44. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/45
Issue
Vol. 19 No. 1 (2021): January - June
Section
Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Journal of TCI is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence, unless otherwise stated. Please read our Policies page for more information.

References

Amoah SK, Sandjo LP, Kratz JM and Biavatti MW. 2016. Rosmarinic acid –Pharmaceutical and clinical aspects. Planta Med. 82: 388–406.

Başer KHC, Kürkçüoğlu M, Demirci, Özek T, Tarımcılar G. 2012. Essential oils of Mentha species from Marmara region of Turkey. J. Essent. Oil Res. 24: 265-72.

Bezerra AC, Barbosa LS, Zura JFC, Oliveira AMF, Azevedo CF. 2019. Structural characterization of mint (Mentha x villosa Huds.) stem and leaf. J. Exp. Agric. Int. 36(2): 1-6.

Chaowuttikul C, Palanuvej C, Ruangrungsi N. 2020. Quantification of chlorogenic acid, rosmarinic acid, and caffeic acid contents in selected Thai medicinal plants using RP-HPLC-DAD. Braz. J. Pharm. Sci. 56(10): 253.

Chotchoungchatchai S, Saralamp P, Jenjittikul T, Pornsiripongse S. 2012. Medicinal plants used with Thai Traditional Medicine in modern healthcare services: A case study in Kabchoeng Hospital, Surin Province, Thailand. J. Ethnopharmacol. 141(1): 193-205.

Ellis BE, Towers GHN. 1970. Biogenesis of rosmarinic acid in Mentha. Biochem. J. 118(2): 291-7.

GBIF Secretariat. 2021. Mentha × cordifolia Opiz ex Frezen. [Internet]. GBIF Backbone Taxonomy. Checklist dataset [cited 2021 Jan. 21]. Available from: https://doi.org/10.15468/39omei accessed.

ICH (Q2R1) Validation of analytical procedures: Text and Methodology. 2005. Geneva: International Conference on Harmonization, International Conference on Harmonization of Technical Requirements for registration of Pharmaceuticals for Human Use.

Maffei M, Gallino M, Sacco T. 1986. Glandular trichomes and essential oils of developing leaves in Mentha viridis lavanduliodora. Planta Med. 52: 187–93.

Naidu AC, Shah GL. 1981. Observation on the cotyledonary stomata and trichomes and their ontogeny in some genera of Lamiaceae. Phyton (Austria). 21: 137-52.

Phumthum M, Srithi K, Inta A, Junsongduang A, Tangjitman K, Pongamornkul W, Trisonthi C, Balslev H. 2017. Ethnomedicinal plant diversity in Thailand. J. Ethnopharmacol. 214(2018): 90-8.

Srirattanakul T, Siripongvutikorn S, Sae-Wong C. 2016. Increasing of bioactive compounds in Mentha cordifolia Opiz., Kitchen mint via ZnSO4 biofortification during plantation. Funct. Foods Health Dis. 6(5): 279-90.

The Government Pharmaceutical Organization. 2013. National List of Essential Medicines (2013): 36.

Tucker AO, Naczi RFC. 2007. Mentha: An overview of its classification and relationships. In: Lawrence BM, editor. Mint: The Genus Mentha. Boca Raton: CRC Press; p. 33

WHO. 2011. Quality Control Methods for Herbal Materials. Geneva. 1-40.

Saric-Kundalic B, Fialova S, Dobes C, Olzant S, Telkelova D, Grancai D, Reznicek G, Saukel J. 2009. Multivariate numerical taxonomy of Mentha species, hybrids, varieties and cultivars. Sci. Pharm. 77: 851-76.