PHYTOCHEMICAL SCREENING TESTS OF MELIENTHA SUAVIS PIERRE AND UROBOTYRA SIAMENSIS HIEPKO EXTRACTS
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Abstract
This research aimed to screen the phytochemical groups of pak-wan pa (Melientha suavis Pierre) and pak-wan mao (Urobotyra siamensis Hiepko). Pak-wan pa was collected from Saraburi and Uthai Thai provinces while pak-wan mao was obtained from Kanchanaburi province, Thailand. The leaves and stems were extracted with four different solvents in order of increasing polarity: hexane, ethyl acetate, methanol and water, using a soxhlet apparatus. Using nonpolar solvents, the hexane and ethyl acetate extracts of pak-wan pa contained alkaloids, carbohydrates, reducing sugars, flavonoids, sterols and tannins. The hexane and ethyl acetate extracts of pak-wan mao contained mostly alkaloids and reducing sugars. The ethyl acetate extracts of pak-wan pa (Uthai Thani) and pak-wan mao contained coumarins. For high polar solvents, the methanol and water extracts of pak-wan pa contained alkaloids, carbohydrates, coumarins, flavonoids, saponins and tannins. The methanol and water extracts of pak-wan mao showed similar components, but without flavonoids. Further study is needed on the separation of these extracts to search for bioactive compounds.
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