A comparative study on spore germination of Diplazum esculentum (Retz.) Sw. “Pako Fern” using different potting media

Xylle Ira F. Guillen; Jeanette Mara P. Tan

Authors

Xylle Ira F. Guillen Department of Biology, Silliman University, Dumaguete City, Negros Oriental, Philippines; Rodolfo B. Gonzales Museum of Natural History, Biology Department, Silliman University, Dumaguete City, Negros Oriental, Philippines
Jeanette Mara P. Tan Rodolfo B. Gonzales Museum of Natural History, Biology Department, Silliman University, Dumaguete City, Negros Oriental, Philippines

Keywords:

Spore germination, Diplazium esculentum, potting media

Abstract

Diplazium esculentum is an economically important species of fern under the family of Athyriaceae, commonly known as the vegetable fern or Pako (Semwal et al., 2021). Studies showed that D. esculentum is an excellent source of food and phytochemical compounds. Many methods and techniques have been utilized in the propagation of ferns. However, no prior studies have delved into the propagation of spores in soil media for D. esculentum, thus this study investigated the spore development of Diplazium esculentum using various potting media. The objectives of this study were to compare and determine which potting media yields the highest growth percentage of D. esculentum and to identify the duration of germination of D. esculentum spores until the gametophyte stage. Five substrates were utilized for the experiment, namely, control group with regular potting mix, carbonized rice hull, coco peat, peat moss, and dried tree fern trunks. Evident growth resulted in the potting media containing carbonized rice hull with 12.21% growth and coco peat with 3.91% growth. No gametophyte growth occurred in media containing dried tree fern trunks and peat moss. Results also showed the importance of soil pH ranging from 5–6 to the spore germination of D. esculentum. Analysis of variance showed that there was a significant difference between the gametophyte growth.

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