Trends on Biocontrol Potential of Metarhizium: A Bibliometric Analysis
Keywords:
Biological control, Entomopathogenic fungi, Integrated pest management, Sustainable agricultureAbstract
Metarhizium is widely recognized as an entomopathogenic fungus with a natural ability to control insects and pests. It has the dual ability to act as a biopesticide and a significant plant growth promoter. This bibliometric analysis was conducted to study global research trends on Metarhizium biocontrol from 2001 to 2024, based on the 772 publications indexed in the Scopus database. VOSviewer and the R Bibliometrix package have been used to examine publication trends, citation patterns, influential authors, journals, institutions, and international collaborations. The results show consistent research productivity with an annual growth rate of 7.6%, which has significantly increased after 2015. The analysis revealed that the highly cited studies are focused on host-pathogen interactions, comparative genomics, and ecological safety of Metarhizium species. Countries like China, Brazil, and the United States are the global centers, while India, Spain, and Kenya are the emerging contributors to the Metarhizium biocontrol research. In recent years, the research themes have evolved from taxonomy and pathogenicity studies (2001–2005) to molecular biology, applied pest management for integrated pest management strategies. The analysis reveals strong global progress, however, gaps remain at the stages of formulation stability, field-level trials under diverse climatic conditions, and non-target ecological safety. The integration of advanced genomics with formulation technology and improving international collaborations are vital to enhancing the practical use of Metarhizium species for eco-friendly biocontrol. This study presents the first comprehensive bibliometric mapping of biocontrol research using Metarhizium species over a 24-year period, providing valuable insights for researchers, policymakers, and industry stakeholders to inform future innovations in biological pest control.
References
AbdelGhany TM. 2015 – Entomopathogenic fungi and their role in biological control. OMICS Group eBooks, Foster City, CA, USA
Aria M, Cuccurullo C. 2017 – Bibliometrix: An R-tool for comprehensive science mapping analysis. Journal of Informetrics 11, 959–975.
Ayaz M, Li CH, Ali Q, Zhao W, et al. 2023 – Bacterial and fungal biocontrol agents for plant disease protection: Journey from lab to field, current status, challenges, and global perspectives. Molecules 28(18), 6735.
Bamisile BS, Dash CK, Akutse KS, Keppanan R, et al. 2018 – Fungal endophytes: Beyond herbivore management. Frontiers in Microbiology 9, 544.
Batta YA. 2016 – Recent advances in formulation and application of entomopathogenic fungi for biocontrol of stored-grain insects. Biocontrol Science and Technology 26(9), 1171–1183.
Bidochka MJ, Kamp AM, Lavender TM, Dekoning J, et al. 2001 – Habitat association in two genetic groups of the insect-pathogenic fungus Metarhizium anisopliae: Uncovering cryptic species? Applied and Environmental Microbiology 67(3), 1335–1342.
Bischoff JF, Rehner SA, Humber RA. 2009 – A multi-locus phylogeny of the Metarhizium anisopliae lineage. Mycologia 101(4), 512–530.
Boaventura H, Quintela E. 2025 – The multifunctionality of the fungus Metarhizium spp. and its use in Brazilian agriculture. Bragantia. (Accessed on January 10, 2025).
Burnham JF. 2006 – Scopus database: A review. Biomedical Digital Libraries 3(1), 1–8.
Butt TM, Jackson C, Magan N. 2001 – Fungi as biocontrol agents: Progress, problems and potential. CABI Publishing. (Accessed on January 10, 2025).
Chaudhary PJ, BL R, Patel HK, Mehta PV, et al. 2023. Plant growth promoting potential of entomopathogenic fungus Metarhizium pinghaense AAUBC M26 under elevated salt stress in tomato. Agronomy 13(6), 1577.
Donthu N, Kumar S, Mukherjee D, Pandey N, et al. 2021 – How to conduct a bibliometric analysis: An overview and guidelines. Journal of Business Research 133, 285–296.
Du Y, Li J, Chen S, Xia Y, Jin K. 2024 – Pathogenicity analysis and comparative genomics reveal the different infection strategies between the generalist Metarhizium anisopliae and the specialist Metarhizium acridum. Pest Management Science 80(2), 820–836.
Elsevier. 2025 – Scopus: Abstract and citation database [Database]. Elsevier. Retrieved August 19, 2025, from https://www.scopus.com (Accessed on January 10, 2025).
Fang W, St. Leger RJ. 2012 – Enhanced UV resistance and improved killing of Malaria mosquitoes by photolyase transgenic entomopathogenic fungi. PLOS ONE 7(8), e43069.
Fang W, Vega-Rodríguez J, Ghosh AK, Jacobs-Lorena M, et al. 2011 – Development of transgenic fungi that kill human malaria parasites in mosquitoes. Science 331(6020), 1074–1077.
Gao Q, Jin K, Ying SH, Zhang Y, et al. 2011 – Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M. acridum. PLoS Genetics 7(1), e1001264.
Geremew D, Shiberu T, Leta A. 2024 – Evaluation of endophytic colonization and establishment of entomopathogenic fungi against Tuta absoluta (Lepidoptera: Gelechiidae) in tomato plants. F1000Research 13, 800.
Hu G, St. Leger RJ. 2002 – Field studies using a recombinant mycoinsecticide (Metarhizium anisopliae) reveal that it is rhizosphere competent. Applied and Environmental Microbiology 68(12), 6383–6387.
Hu X, Xiao G, Zheng P, Shang Y, et al. 2014 – Trajectory and genomic determinants of fungal-pathogen speciation and host adaptation. Proceedings of the National Academy of Sciences 111(47), 16796–16801.
Inglis GD, Goettel MS, Butt TM, Strasser H. 2001 – Use of hyphomycetous fungi for managing insect pests. In: T. M. Butt, C. Jackson, N. Magan (Eds.), Fungi as biocontrol agents: Progress, problems and potential (pp. 23–69). CABI Publishing. (Accessed on January 10, 2025).
Jaber LR, Ownley BH. 2018 – Can we use entomopathogenic fungi as endophytes for dual biological control of insect pests and plant pathogens? Biological Control 116, 36–45.
Jaronski ST. 2009 – Ecological factors in the inundative use of fungal entomopathogens. BioControl 55(1), 159–185.
Joo JH, Hussein KA. 2022 – Biological control and plant growth promotion properties of volatile organic compound-producing antagonistic Trichoderma spp. Frontiers in Plant Science 13, 897668.
Karthi S, Vasantha-Srinivasan P, Senthil-Nathan S, Han YS, et al. 2024 – Entomopathogenic fungi promising biocontrol agents for managing lepidopteran pests: Review of current knowledge. Biocatalysis and Agricultural Biotechnology 58, 103146.
Kobmoo N, Mongkolsamrit S, Khonsanit A, Cedeño-Sanchez M, et al. 2024 – Integrative taxonomy of the Metarhizium anisopliae species complex based on phylogenomics combined with morphometrics, metabolomics and virulence data. IMA Fungus 15, 30
Kortsinoglou AM, Wood MJ, Myridakis AI, Andrikopoulos M, et al. 2024 – Comparative genomics of Metarhizium brunneum strains V275 and ARSEF 4556: unraveling intraspecies diversity. G3 (Bethesda, Md.) 14(10), jkae190.
Lotka AJ. 1926 – The frequency distribution of scientific productivity. Journal of the Washington Academy of Sciences 16(12), 317–324.
Lovett B, St. Leger RJ. 2017 – Genetically engineering better fungal biocontrol agents. Pest Management Science 73(1), 10–17.
Magan N, Medina A. 2021 – Climate change and resilience of biological control agents. In: how research can stimulate the development of commercial biological control against plant diseases (pp. 83–93). Cham: Springer International Publishing.
Mesquita E, Hu S, Lima TB, Golo PS, et al. 2023 – Utilization of Metarhizium as an insect biocontrol agent and a plant bioinoculant with special reference to Brazil. Frontiers in Fungal Biology 4, 1276287.
Meyling NV, Eilenberg J. 2007 – Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: Potential for conservation biological control. Biological Control 43(2), 145–155.
Moher D, Liberati A, Tetzlaff J, Altman DG, et al. 2009 – Preferred reporting items for systematic reviews and meta-analyses: The PRISMA statement. PLoS Medicine 6(7), e1000097.
Mongkolsamrit S, Khonsanit A, Thanakitpipattana D, Tasanathai K, et al. 2020 –Revisiting Metarhizium and the description of new species from Thailand. Studies in Mycology 95, 171–251.
Ninkov A, Frank JR, Maggio LA. 2022 – Bibliometrics: Methods for studying academic publishing. Perspectives on Medical Education 11(3), 173–176.
Ortiz-Urquiza A, Keyhani NO. 2013 – Action on the surface: Entomopathogenic fungi versus the insect cuticle. Insects 4(3), 357–374.
Peng ZY, Huang ST, Chen JT, Li N, et al. 2022 – An update of a green pesticide: Metarhizium anisopliae. All Life 15(1), 1141–1159.
Quesada Moraga E, Garrido Jurado I, González Mas N, Yousef Yousef M. 2023 – Ecosystem services of entomopathogenic ascomycetes. Journal of Invertebrate Pathology 201, 108015.
San-Blas E. 2013 – Progress on entomopathogenic nematology research: A bibliometric study of the last three decades: 1980–2010. Biological Control 66(2), 102–124.
Scopus database. 2025 – Available at: www.scopus.com, (Accessed on August 19, 2025).
Segata N, Izard J, Waldron L, Gevers D, et al. 2011 – Metagenomic biomarker discovery and explanation. Genome Biology 12(6), R60.
Sharma R, Sharma P. 2021 – Fungal entomopathogens: A systematic review. Egyptian Journal of Biological Pest Control 31, 57.
Sullivan CF. 2022 – A review of commercial Metarhizium- and Beauveria-based products in the USA. Insects 13(2), 158.
Valero-Jiménez CA, Wiegers H, Zwaan BJ, Koenraadt CJM, et al. 2016 – Genes involved in virulence of the entomopathogenic fungus Beauveria bassiana. Journal of Invertebrate Pathology 133, 41–49.
Van Eck NJ, Waltman L. 2010 – VOSviewer: A computer program for bibliometric mapping. Scientometrics 84(2), 523–538.
Wang C St, Leger RJ. 2007 – The MAD1 adhesin of Metarhizium anisopliae links adhesion with blastospore production and virulence to insects, and the MAD2 adhesin enables attachment to plants. Eukaryotic Cell 6(5), 808–816.
Wang J, Weng Q, Zhang K, Hu Q. 2023 – Binding proteins of destruxin A from Metarhizium against insect cell. BMC Microbiology 23(1), 96.
Wilson K, Thomas MB, Blanford S, Doggett M, et al. 2002 – Coping with crowds: Density-dependent disease resistance in desert locusts. Proceedings of the National Academy of Sciences of the United States of America 99(8), 5471–5475.
Yapa AT, Thambugala KM, Samarakoon MC, de Silva N. 2025 – Metarhizium species as bioinsecticides: potential, progress, applications & future perspectives. New Zealand Journal of Botany 63(2–3), 439–461.
Yapa PAB, Silva R, Jayasena H. 2025 – Global trends and applications of Metarhizium in sustainable pest management. Journal of Applied Microbiology 138(2), 511–526.
Yapa RH, Silva GA, Perera S. 2025 – Multifunctional role of Metarhizium anisopliae in crop health and pest management. Journal of Fungi 11(3), 356.
Yu Q, Liu YX, Liu ZY. 2011 – Advances in classification of Metarhizium. Guizhou Agricultural Sciences. Available at: https://www.cabidigitallibrary.org/doi/full/10.5555/20123077140 (Accessed on August 11, 2025).
Yuan Z, Shen Q, Yu K, Liu Y, et al. 2025 – Half-Century Scientometric Analysis: Unveiling the Excellence of Fungi as Biocontrol Agents and Biofertilisers. Journal of Fungi 11(2), 117.
Zhou W, Li M, Achal V. 2025 – A comprehensive review on environmental and human health impacts of chemical pesticide usage. Emerging Contaminants 11(1), 100410.
Zimmermann G. 2007 – Review on safety of the entomopathogenic fungus Metarhizium anisopliae. Biocontrol Science and Technology 17(9), 879–920.
