THE CHOLESTEROL ESTERASE INHIBITION AND TOTAL PHENOLIC CONTENT OF AQUEOUS EXTRACT OF TRIPHALA AND MODIFIED TRIPHALA FORMULAS

Article Sidebar

PDF
Published: Oct 5, 2023
Keywords:
Triphala total phenolic content cholesterol esterase enzyme inhibition

Main Article Content

Pathamaporn Pathompak
The Herbal Medicinal Products Research and Development Center (Cooperation between Rangsit University, Harbin Institute of Technology, and Heilongjiang University of Chinese Medicine), Faculty of Pharmacy, Rangsit University, Muang, Pathum Thani 12000, Thailand
Laksana Charoenchai
The Herbal Medicinal Products Research and Development Center (Cooperation between Rangsit University, Harbin Institute of Technology, and Heilongjiang University of Chinese Medicine), Faculty of Pharmacy, Rangsit University, Muang, Pathum Thani 12000, Thailand
Chaowalit Monton
The Herbal Medicinal Products Research and Development Center (Cooperation between Rangsit University, Harbin Institute of Technology, and Heilongjiang University of Chinese Medicine), Faculty of Pharmacy, Rangsit University, Muang, Pathum Thani 12000, Thailand

Abstract

Hypercholesterolemia is the important public health problems. Triphala is traditional medicinal formula that is recognized to demonstrate the antioxidative activity and the blood cholesterol reducing property. It composes of fruits of three herbal plants, Terminalia bellirica, Terminalia chebula and Phyllanthus emblica. The objective of this study is to investigate the effect of aqueous extract of Triphala and modified Triphala formulas on cholesterol esterase inhibition and total phenolic content. Each formula was extracted in duplicate called extraction 1 and extraction 2. The results indicated that modified Triphala formula 2 (MT.F.2), which contained T. bellirica, T. chebula and P. emblica in 12:8:4 weight ratio,  expressed the highest total phenolic content at 514.4±5.1 µg GAE/mg plant extract for extraction 1 and 516.3±22.9 µg GAE/mg plant extract for extraction 2, respectively. The cholesterol esterase (CEase) was inhibited only 4-11% by aqueous extract of Triphala and modified Triphala formulas at concentration of 2.0 mg/ml. The MT.F.2 manifested the highest percent inhibitory values of 8.4±0.4 for extraction 1 and 10.6±0.5 for extraction 2, respectively. In conclusion, total phenolic content involve in the proportion of T. bellirica in formula. On the contrary, the inhibition of CEase seemed not related to the amount of each herbal plant in formula.  

Article Details

How to Cite
1.
Pathompak P, Charoenchai L, Monton C. THE CHOLESTEROL ESTERASE INHIBITION AND TOTAL PHENOLIC CONTENT OF AQUEOUS EXTRACT OF TRIPHALA AND MODIFIED TRIPHALA FORMULAS. Interprof J Health Sci [Internet]. 2023 Oct. 5 [cited 2024 Nov. 21];13(2):25-30. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/117
Issue
Vol. 13 No. 2 (2015): July - December
Section
Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Journal of TCI is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence, unless otherwise stated. Please read our Policies page for more information.

References

Adisakwattana S, Intrawangso J, Hemrid A, Chanathong B, Mäkynen K. 2012. Extracts of edible plants inhibit pancreatic lipase, cholesterol esterase and cholesterol micellization, and bind bile acids. Food Technol. Biotech. 1: 11-16.

Castelli WP. 1984. Epidemiology of coronary heart-disease-the Framingham-study. Am. J. Med. 76: 4-12.

Chiou SY, Lai GW, Lin LY, Lin G. 2006. Kinetics and mechanisms of cholesterol esterase inhibition by cardiovascular drugs in vitro. Indian J. Biochem. Bio. 43(1): 52-55.

Elahi MM, Kong YX, Matata, BM. 2009. Oxidative stress as a mediator of cardiovascular disease. Oxid. Med. Cell. Longev. 2: 259-269.

Heidrich JE, Contos LM, Hunsaker LA, Deck LM, Vander JDL. 2004. Inhibition of pancreatic cholesterol esterase reduces cholesterol absorption in the hamster. BMC Pharmacol. 4: 1-9.

Howles PN, Carter CP, Hui DY. 1996. Dietary free and esterified cholesterol absorption in cholesterol esterase (bile salt-stimulated lipase) gene-targeted mice. J. Biol. Chem. 271(12): 7196-7202.

Naik GH, Priyadarsini KI, Bhagirathi RG, Mishra B, Mishra KP, Banavalikar MM, et al. 2005. In vitro antioxidant studies and free radical reactions of Triphala, an ayurvedic formulation and its constituents. Phytother. Res.19: 582-586.

Naik GH, Priyadarsini KI, Mohan H. 2006. Free radical scavenging reactions and phytochemical analysis of Triphala, an ayurvedic formulation. Curr. Sci. India. 90(8): 1100-1105.

Pioruńska-Stolzmann M, Pioruńska-Mikołajczak A. 2001. The influence of simvastatin on lipase and cholesterol esterase activity in the serum of men with coronary heart disease. Pharmacol. Res. 43(4): 359-362.

Russell JRLH, Mazzio E, Badisa RB, Zhu ZP, Agharahimi M, Millington DJ, et al. 2011. Differential cytotoxicity of Triphala and its phenolic constituent gallic acid on human prostate cancer LNCap and normal cells. Anticancer Res. 31(11): 3739–3745.

Sabu MC, Kuttan R. 2002. Anti-diabetic activity of medicinal plants and its relationship with their antioxidant property. J. Ethnopharmacol. 81: 155-160.

Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Method. Enzymol. 299: 152-178.

Weststrate JA, Meijer GW. 1998. Plant sterol-enriched margarines and reduction of plasma total- and LDL-cholesterol concentrations in normocholesterolaemic and mildly hypercholesterolaemic subjects. Eur. J. Clin. Nutr. 52(5): 334-343.