THE EFFECT OF EMPTY BOTTLE STIMULATION ON THE BEHAVIORS OF RATS AND EVALUATION OF OPEN-FILED TEST SYSTEM

Main Article Content

Xiaonan Ye
Thanes Fuangfoo
Tingli Li

Abstract

To clarify the relationship between emotional stress and anxiety. Method: Use the empty bottle stimulation to establish the model of chronic emotional stress. Rat's serum corticosterone concentration were measured by ELISA. Then researchers observed behavioural changes of model rats and evaluation of open-filed test system. Result: Compared with the control group, model group rats’s attacking, exploring behavior significantly increased (P < 0.01), serum corticosterone concentration significantly increased (P < 0.01), in the centre area entry times and duration were significantly reduced (P < 0.05). Conclusion: The empty bottle stimulus used as a model of chronic emotional stress, can make the HPA axis activation and make rats’s serum corticosterone levels increased and can change the the behavior of rats, can be used as anxiety model for drug research.

Article Details

How to Cite
1.
Ye X, Fuangfoo T, Li T. THE EFFECT OF EMPTY BOTTLE STIMULATION ON THE BEHAVIORS OF RATS AND EVALUATION OF OPEN-FILED TEST SYSTEM. Interprof J Health Sci [Internet]. 2023 Oct. 5 [cited 2024 Dec. 6];13(2):75-9. Available from: https://li05.tci-thaijo.org/index.php/IJHS/article/view/124
Section
Research Articles

References

Blanchard RJ, Nikulina JN, Sakai RR, McKittrick C, McEwen B, Blanchard DC. 1998. Behavioral and endocrine change following chronic predatory stress. Physiol Behav. 63 (4): 561–9. doi: 10.1016/S0031-9384(97)00508-8

Croiset G, Heijnen CJ, Veldhuis HD, de Wied D, Ballieux RE. 1987. Modulation of the immune response by emotional stress. Life Sci. 40: 775-82. doi: 10.1016/0024-3205(87)90305-5

Dietrich M, Abbott KV, Gartner-Schmidt J, Rosen CA. 2006. The frequency of perceived stress, anxiety, and depression in patients with common pathologies affecting voice. J of Voice. 22 (4): 472–88. doi: 10.1016/j.jvoice.2006.08.007

Giddens CL, Barron KW, Byrd-Craven J. et al. 2013. Vocal indices of stress: a review. J Voice. 27 (3): 390. e21-390.e29. doi: 10.1016/j.jvoice.2012.12.010

Hacquemand R, Choffat N, Jacquot L, Brand G. 2013. Comparison between low doses of TMT and cat odor exposure in anxiety- and fear-related behaviors in mice. Behavioural Brain Research. 238: 227-31.

Jia M, Smerin SE, Zhang L, et al. 2015. Corticosterone mitigates the stress response in an animal model of PTSD. J. Psychiatric Res. 60: 29-39. doi: 10.1016/j.jpsychires.2014.09.020

Kott JM, Mooney-Leber SM, Shoubah FA, Brummelte S. 2016. Effectiveness of different corticosterone administration methods to elevate corticosterone serum levels, induce depressive-like behavior, and affect neurogenesis levels in female rats. Neuroscience. 312: 201–14. doi: 10.1016/ j.neuroscience.2015.11.006

Lin WJ, Wang WW, Shao F. 2003. New emotional stress model: Effects of chronic emotional stress on rat’s behaviors, neuroendocrine and immune responses. Chin Sci Bull. 48 (15): 1565-68.

Lucassen PJ, Pruessner J, Sousa N, Almeida OFX, Van Dam AM, Rajkowska G, Swaab DF, et al. 2014. Neuropathology of stress. Acta Neuropathol. 127: 109-35. doi: 10.1007/s00401-013-1223-5

Paolo SD, Brain P, Willner P. 1994. Effects of chronic mild stress on performance in behavioural tests relevant to anxiety and depression. Physiology & Behavior. 56 (5): 861-867. doi: 10.1016/0031-9384(94)90316-6

Ramos A, Pereira E, Martins GC, Wehrmeister TD, Izı´dio GS André R. 2008. Integrating the open field, elevated plus maze and light/dark box to assess different types of emotional behaviors in one single trial. Behavioural Brain Research. 193: 277–88. doi: 10.1016/j.bbr.2008.06.007

Veissier I, Boissy A. 2007. Stress and welfare: Two complementary concepts that are intrinsically related to the animal's point of view. Physiology & Behavior. 92: 429-33. doi: 10.1016/j.physbeh.2006.11.008