THE EFFECT OF EMPTY BOTTLE STIMULATION ON THE BEHAVIORS OF RATS AND EVALUATION OF OPEN-FILED TEST SYSTEM
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Abstract
To clarify the relationship between emotional stress and anxiety. Method: Use the empty bottle stimulation to establish the model of chronic emotional stress. Rat's serum corticosterone concentration were measured by ELISA. Then researchers observed behavioural changes of model rats and evaluation of open-filed test system. Result: Compared with the control group, model group rats’s attacking, exploring behavior significantly increased (P < 0.01), serum corticosterone concentration significantly increased (P < 0.01), in the centre area entry times and duration were significantly reduced (P < 0.05). Conclusion: The empty bottle stimulus used as a model of chronic emotional stress, can make the HPA axis activation and make rats’s serum corticosterone levels increased and can change the the behavior of rats, can be used as anxiety model for drug research.
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