การคัดแยก จำแนกและศึกษาคุณสมบัติของเชื้อ Streptomyces sp. SAURU59  ที่ใช้กำจัดเชื้อราก่อโรครากเน่าโคนเน่าของทุเรียน

Wanwisa   Pirapak; Pattachai  Pinnak; Pilunthana  Thapanapongworakul

Authors

Wanwisa Pirapak Program of Biology, Faculty of Science and Technology, Uttaradit Rajabhat University, Thailand
Pattachai Pinnak Program of Biology, Faculty of Science and Technology, Uttaradit Rajabhat University, Thailand
Pilunthana Thapanapongworakul Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Thailand

Keywords:

root and stem rot disease , durian, antagonistic actinomycete

Abstract

This research aimed to select an actinomycete strain from soil collected in Nan River National Park, Tha Pla District, Uttaradit Province, using the soil dilution plate method and dual culture technique to examine Phytophthora palmivora, the causative agent of durian root and stem rot disease. The research also included the identification of antagonistic actinomycetes and an investigation into the biochemical properties of these actinomycetes. A total of 69 isolates were obtained from the soil, with only isolate SAURU59 found to exhibit the highest efficiency in inhibiting the growth of the fungal pathogen, achieving a 75 percent inhibition rate. Morphological characterization and analysis of the 16S rRNA nucleotide sequence revealed that SAURU59 was gram-positive and had spores formed in a chain-like structure with a spiral and smooth surface. Additionally, the nucleotide sequence of SAURU59 showed similarity to Streptomyces rimosus. This isolate showed the ability to produce cellulase and chitinase enzymes, as well as 30.78 µg/ml of indole-3-acetic acid (IAA) within 10 days.

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