การดื้อยาปฏิชีวนะและความหลากหลายทางพันธุกรรมของ Escherichia coli ที่แยกได้จากมูลสุกรในจังหวัดพะเยา

Nitsara  Boonkerd; Surasak Chaikhiandee

Authors

Nitsara Boonkerd Microbiology and Parasitology, School of Medical Science, University of Phayao
Surasak Chaikhiandee Nutrition and Dietetics, School of Medical Science, University of Phayao

Keywords:

antibiotic resistance, Escherichia coli , swine , rep-PCR

Abstract

Antibiotic resistance is one of the problems of public health worldwide. Currently, the improper use of antibiotics is an important factor in the spread of drug resistance. Therefore, the monitoring of the drug resistance is crucial for the selection of appropriate drugs in swine. The objectives were investigated the resistance patterns and genetic relationships of 166 E. coli isolates that were isolated from adult swine, sick swine and piglet feces in Muang District, Phayao Province. The results showed that 154 E. coli isolates (92.77%) were resistant to all antibiotics used in this study. It was found that the highest resistance rate to ampicillin, which is equal to 80, 80 and 95.7 % in isolates from adult swine, sick swine, and piglets, respectively. From the analysis of resistance patterns of E. coli, it was found 16 resistance patterns. The highest drug resistance pattern that could be found is ampicillin, chloramphenicol, sulfamethoxazole/trimethoprim and tetracycline. The rate of this drug resistance pattern is 20, 23.33 and 39.13 % in isolates from adult swine, sick swine and piglets, respectively. In addition, 36.66, 66.66 and 82.62 % of these groups were resistant to more than three drug types. The genetic variation of E. coli was studied by repetitive extragenic palindromic PCR (rep-PCR). It found 15 rep-PCR patterns (A-O). The most common rep-PCR pattern is the pattern D that found about 43.14 % (22/51 isolates) and indicated the spread of E. coli in pig farms. The results of all showed the resistance situation of E. coli in swine, which is the basic information in the planning, controlling, and prevention of drug resistance. In addition, it can be used as a guideline for the selection of antibiotics in swine.

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