การขยายกำลังการผลิตเอนไซม์โปรติเอสเพื่อประยุกต์ใช้ในการผลิต ถุงมือยางทางการแพทย์ที่ปราศจากโปรตีนภูมิแพ้

Pairote   Wongputtisin; Chantana   Supo

Authors

Pairote Wongputtisin Program in Biotechnology, Faculty of Science, Maejo University
Chantana Supo Program in Biotechnology, Faculty of Science, Maejo University

Keywords:

protease , Bacillus sp. , up-scaling process , allergic protein , latex medical glove

Abstract

Para rubber (Hevea brasiliensis) is an important economic crop of Thailand. However, natural Para rubber latex (NPRL) contains several allergic proteins and remained in medical devices which made from NPRL. Patients and medical staff are harmed by these allergens. Our research group has found that protease from Bacillus sp. bacterium is able to eliminate these allergic proteins in NPRL. However, large quantity of protease is required for using in the industrial scale. This research was subsequently aimed to study the optimal conditions for protease production by Bacillus subtilis MR10 in the 20 L fermenter and the possibility of medical glove production by allergic protein-free NPRL. It was found that the optimal condition for protease production was cultivation at 28-30 ^oC, aeration rate at 1.0 kg/cm² and agitation rate at 150 rpm, leading to 1.21 unit/ml-hr of protease productivity. The obtained protease could eliminate allergic proteins, i.e. Hev b1 and Hev b2, when dot bot technique was carried out. The medical gloves produced from allergic protein free-NPRL exhibited similar thickness, color and surface to those from NPRL, however protease decreased tensile strength of gloves. Therefore, the formula development and optimization for vulcanization process need to be investigated prior application in allergic protein-free glove manufacturing.

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