Effect of Bacillus licheniformis on Growth, Survival Rate and Immune of Giant Freshwater Prawn (Macrobrachium rosenbergii)
Keywords:
giant freshwater prawn , probiotics, Bacillus ssp. , immune systemAbstract
Background and Objectives : Giant freshwater prawn are economic freshwater aquatic species. The intensive prawn culture affects on slow growth and disease outbreaks. Currently, there are biological shrimp farms using microorganism sush as Bacillus spp. for enhance shrimp growth and immune. The objective of this study is to determine effects of Bacillus licheniformis on growth survival rate and immune of Macrobrachium rosenbergii .
Methodology : Prawns (size 5.23±0.007 g) were stocked in 0.52×1×0.42 meters tanks at density 13 prawns/tank (25 prawns/m2). The experiment was Completely Randomized Design (CRD) and divided in to 4 treatments. Each treatment had 3 replications. The first treatment was commercial pelleted feed (control), the 2nd, 3rd and 4th treatments were commercial pelleted feed with B. licheniformis at concentration 1, 5 and 10 g/feed 1 kilograms respectively. The growth, survival rate and immune were recorded during culture for 60 days.
Main Results : Growth and survival rate of prawn in treatment 4, 3 and 2 fed with B. licheniformis were 11.71±0.29, 11.18±0.56, and 10.41±0.71 grams, respectively and significantly higher than the control group (p<0.05). For the prawn immunity, prawn fed with probiotic in treatment 4 had total hemocyte count and phagocytosis activity better than control group (p<0.05).
Conclusions : Prawn fed with diet containing B. licheniformis at concentration 10 g/feed 1 kilograms was able to enhance growth, survival rates and immune than the control group.
References
Abarike, E. D., Cai, J., Lua, Y., Yu, H., Chen, L., Jian, J., Tang, J., Jun, L., & Kuebutornye F. K.A. (2018). Effects of a commercial probiotic BS containing Bacillus subtilis and Bacillus licheniformis on growth, immune response and disease resistance in Nile tilapia, Oreochromis niloticus. Fish & Shellfish Immunology, 82, 229–238
Abdollahi-Arpanahi, D., Soltani, E., Jafaryan, H., Soltani, M., Naderi-Samani, M., & Campa-Córdovae, A. I. (2018). Efficacy of two commercial and indigenous probiotics, Bacillus subtilis and Bacillus licheniformis on growth performance, immuno-physiology and resistance response of juvenile white shrimp (Litopenaeus vannamei). Aquaculture, 496(1), 43-49
Amoah, K., Dong, X., Tan, B., Zhang, S., Chi, S., Yang, Q., Liu, H., Yang, Y., & Zhang, H. (2020). Administration of probiotic Bacillus licheniformis induces growth, immune and antioxidant enzyme activities, gut microbiota assembly and resistance to Vibrio parahaemolyticus in Litopenaeus vannamei. Aquaculture Nutrition, 26(5), 1-19.
Andriani, Y., Safitri, R., Rochima, E., & Fakhrudin, S. D. (2017). Characterization of Bacillus subtilis and B. licheniformis potentials as probiotic bacteria in Vanamei shrimp feed (Litopenaeus vannamei Boone, 1931). Nusantara Bioscience, 9(2), 188-192
Arguelles-Arias, A., Ongena, M., Halimi, B., Lara, Y., Brans, A., Joris, B., & Fickers, P. (2009). Bacillus amyloliquefaciens GA1 as a source of potent antibiotics and mother secondary metabolites for biocontrol of plant pathogens. Microbial Cell Factories, 8(63), 1-12.
Boonyapakdee, A., Boonnom, S., Silarudee, K., Silarudee, S., & Limheng, K. (2021). The effects of probiotics supplementation on growth and survival rate of blue swimming crablets (Portunus pelagicus). Khon kaen Agriculture Journal Suppl, 49(1), 140-144 (in Thai)
Chen M., Chen, X.Q., Tian, L.X., Liu, Y.J. & Niu, J. (2020). Beneficial impacts on growth, intestinal health, immune responses and ammonia resistance of pacific white shrimp (Litopenaeus vannamei) fed dietary synbiotic (mannan oligosaccharide and Bacillus licheniformis). Aquaculture Reports, 17-100408
Cladera-Olivera, F., Caron, G.R., & A. Brandelli. (2004). Bacteriocin-like substance production by Bacillus licheniformis strain P40. Microbiology, 38(4), 251-256
Fishery Statistics Group. (2023). Statistics of freshwater aquaculture production 2022. Fisheries development policy and planning division. Department of fisheries. No. 9/2023. (in Thai)
Gobi, N., Vaseeharan B., Chenb J.C., Rekhaa, R., Vijayakumar S., Anjugam, M., & Iswarya, A. (2018). Dietary supplementation of probiotic Bacillus licheniformis Dahb1 improves growth 2 performance, mucus and serum immune parameters, antioxidant enzyme activity as well as 3 resistances against Aeromonas hydrophila in tilapia Oreochromis mossambicus. Fish & Shellfish Immunology, 74, 501-508
Itami, T., Takahashi, Y., Tsuchihira, E., Igasu , H., & Kondo, M. (1994). Enhancement of disease resistance of Kuruma prawn Penaeus japonicus and increase in phagocytic activity of prawn hemocytes after oral administration of B-1,3-glucan (Schizophyllan). In Proceeding of the Third Asian Fisheries Forum Singapore. (pp. 375-378). The Asian Fisheries Society Manila, Philippines.
Jueliang, P. (2011). Application of Spore-forming Bacteria for Controlling Pathogenic Bacteria Vibrio harveyi in Litopenaeus vannamei Culture. Master dissertation. Bangkok: Kasetsart University. (in Thai)
Kim, Y. O., Mahboob, S., Viayaraghavan, P., Biji, D., Al-Ghanim, Al-Misned, K. A., F., & Kim, H. (2020). Growth promoting activity of Penaeus indicus by secondary metabolite producing probiotic bacterium Bacillus subtilis isolated from the shrimp gut. Journal of King Saud University – Science, 32(2), 1641-1646.
Labaiden, M., Nonwachai, T., Boonprasert, W., & Longprig, T. (2022). Effect of probiotics on growth, survival Rate and resistance to Aeromonas sp. in rearing of giant freshwater prawn (Macrobrachium rosenbergii). Burapha Science Journal, 28(2), 1285-1302. (in Thai)
Li, K., Zheng,T., Tian, Y., Xi, F., Yuan, J., Zhang , G., & Hong, H.. (2 0 0 7). Beneficial effects of Bacillus licheniformis on the intestinal microflora and immunity of the white shrimp, Litopenaeus vannamei. biotechnology letter, 29, 525-530
Liu C-H., Chiu C-H., Wang S-W., & Cheng W. (2012). Dietary administration of the probiotic, Bacillus subtilis E20, enhances the growth, innate immune responses, and disease resistance of the grouper (Epinephelus coioides). Fish & Shellfsh Immunology, 33(4), 699–706
Mamdoh, Y.J., Idres, A.A., Mamdouh, A.H., & Sambhu, C. (2019). Probiotics as alternative control measures in shrimp aquaculture: A review. Journal of Applied Biology & Biotechnology, 7(3), 69-77.
Monier,M. N., Kabary, H., Elfeky, A., Saadony, S., Abd El Hamed, N. N. B., Eissa, M. E. H., & Eissa, E.S.H. (2023). The effects of Bacillus species probiotics (Bacillus subtilis and B. licheniformis) on the water quality, immune responses, and resistance of white leg shrimp (Litopenaeus vannamei) against Fusarium solani infection. Aquaculture International, 31(6), 3437–3455
Nonwachai, T. (2012). Effect of Dissolved oxygen, ammonia and pH level on feed intake, growth, survival, non-specific immune characteristic of Pacific white shrimp (Litopenaeus vavvamei) and challenged with Vibrio harveyi. Thesis of Doctor of philosophy (Fisheries Science). Kasetsart university. Bangkok. (in Thai)
Petjul, K., Tankrathok, A.& Chaisemsaeng, P. (2019). Screening of Protease Producing Probiotic Bacteria of Giant Freshwater Prawn (Macrobrachium rosenbergii de Man) on Pond Culture in Kalasin Province. Koch Cha Sarn Journal of Science, 40(2), 31-44. (in Thai)
Phinyo, M., Thinjan, P., & Inyawiler, W. (2018). Use of Coconut Residue and Bacillus licheniformis in Feed for Silver Barb. Burapha Science Journal, 23(2), 1165-1177. (in Thai)
Pimmadee, S. (2023). Situation of giant freshwater prawn and products in the first 6 months of 2023. Fisheries Economy group, Fisheries development policy and planning division. Department of fisheries. (in Thai)
Promnuan, K., & Kiriratnikhom, S. (2018). Effect of different levels of Bacillus licheniformis supplements in diets on growth performance, feed utilization and intestinal bacteria of hybrid red tilapia. Thaksin University Journal, 21(2), 43-50. (in Thai)
Salaipeth, L. (2008). Development of probiotic product for application in white shrimp (Litopenaeus vannamei). The master thesis of science in biotechnology, Prince of Songkla University.
Santos, R. A., Oliva-Teles, A., Pousão-Ferreira, P., Jerusik, R., Saavedra, M.J., Enes, P., & Serra, C. R. (2021). Isolation and characterization of fish-gut Bacillus spp. as source of natural antimicrobial compounds to fight aquaculture bacterial diseases. Marine Biotechnology, 23(2), 276-293.
Supamattaya, K., Raungsri, J., Kiriratnikom, S., & Suanyuk, N. (2020). The immune system in black tiger shrimp, Penaeus monodon Fabricius :VI, Effect of diseases on immuno-physiological function and blood components in black tiger shrimp (Penaeus monodon Fabricius) Songklanakarin Journal of Science and Technology, 22 (Suppl.), 615-621 (in Thai)
Vinoj, G., Vaseeharan, B., DavidJayaseelan, B., Rajakumaran, P., & Ravi, C. (2013). Inhibitory effects of Bacillus licheniformis (DAB1) and Pseudomonas aeruginosa (DAP1) against Vibrio parahaemolyticus isolated from Fenneropenaeus indicus. Aquaculture, 21, 1121-1135.
Wannapat, N. (2019). Guidebook to Freshwater prawn breeding under biosecurity systems. Freshwater Aquaculture Research and Development Division: Department of Fisheries. (in Thai)
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